FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242887
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16960.6982670953982717No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12650.5208183229238288No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11950.4919983366750794No Hit
TCCTTATACCATATAGAAAAATCAACCCAAGATGGATTAAAGACTTAAAT9960.41006723291077746No Hit
GTATAATATTTCCTAGGTTATCTTCCAGGGGCTTTATAGCTTTAGGTTTA9200.3787769621264209No Hit
GTGCTGGGATGAACATGTGAGTGCATGTGTCTTTTTGGGAGAACAGTTTA7780.32031356145038636No Hit
CTTCTACTGTTCCTGTCTTTAACCCTTATTGGAAAACACCCTCTTTTCCT7220.29725757245138684No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5770.23755902950754879No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5430.2235607504724419No Hit
GCCTAATCAGTTATCTTAGCACCATTTATTAAATAGAGAATCCTTTCCCC5360.220678751847567No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5080.2091507573480672No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5000.20585704463392443No Hit
GTTTTTAATAAGTAGTGCTGGGAAAACTGGATATCCATATGCAGAAGAAT4840.19926961920563885No Hit
GCCTAATCCAATGTCCTGGAGCCTTTTCCCATTTTAAAAATTAGTTTCAT4760.19597590649149602No Hit
GATTAGGATGGTATAATATTTCCTAGGTTATCTTCCAGGGGCTTTATAGC4670.1922704796880854No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT4560.18774162470613906No Hit
GGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGG4460.18362448381346058No Hit
GCCTAATCCAGTTTTATTCCAGTAGTTTCATAGTTTCAGGTATTAGATTT4400.18115419927785348No Hit
ACATAATAGAGAACCCAGAAATAAGTCCACACACCTACAACCATCTGATC4270.17580191611737145No Hit
GCCTAATCCAATGTTTTGAAGATTTTCCCCAGTGTTTTCTTTTAGTGGTT4030.1659207779749431No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4020.16550906388567524No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC3960.16303877935006814No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG3820.15727478210031826No Hit
GGGTTTGTATAGTTTTGAGTTTTACATTTAAGTCTTTAATCCATCTTCGG3690.15192249893983623No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3570.14698192986862202No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG3540.1457467876008185No Hit
GTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGTGTTTCTTCAAAA3440.14162964670814002No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCC3340.1375125058154615No Hit
GTGCTGGTGATAACTGGCTAGCCATATGCCAAAGATTGAACTAGATCCCT3320.1366890776369258No Hit
GGTGATAACTGGCTAGCCATATGCCAAAGATTGAACTAGATCCCTTCCTT3270.13463050719058656No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA3170.13051336629790808No Hit
TCCTCAGGCCGTGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG3160.13010165220864023No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC3130.12886650994083668No Hit
TCTTTACACTGCTCCTATAAATTGAATTATTATGTACTCTTTTGAATCTA3100.12763136767303312No Hit
GCCTGAGGATGCGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT3090.12721965358376527No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3050.1255727972266939No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTTATTGGAAAACACCCTCTTTTC3020.12433765495889035No Hit
GACCAATGGAACAAAATACAGAACCCAGAAATAAAGCCCTGGACCTACAG3000.12351422678035465No Hit
CTATATCACAATGTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGT2950.12145565633401541No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG2910.119808799976944No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA2870.1181619436198726No Hit
GTCATATGATTAGGATGGTATAATATTTCCTAGGTTATCTTCCAGGGGCT2840.11692680135206907No Hit
GCACGGGACCCTGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG2840.11692680135206907No Hit
GTCCTGAATGGTATTGCCTAGGTTTTCTTCTAGGGTTTTTATGGTTTTAG2620.10786909138817638No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA2540.1045753786740336No Hit
GAACATAATAGAGAACCCAGAAATAAGTCCACACACCTACAACCATCTGA2500.10292852231696221No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA2500.10292852231696221No Hit
ATTATGTACTCTTTTGAATCTAGCTTCTTTCTCTCAGCTTAATTTTTTGA2470.10169338004915866No Hit
GGTACAAAAACAGACACATAAACCATTGGAACAGAATAGAGAGCCCAGAA2430.10004652369208727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)