FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242887
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA15020.618394562080309No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13960.574752868617917No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11910.490351480318008No Hit
GTGCTGGGATGAACATGTGAGTGCATGTGTCTTTTTGGGAGAACAGTTTA9260.381247246662028No Hit
GTATAATATTTCCTAGGTTATCTTCCAGGGGCTTTATAGCTTTAGGTTTA8540.3516038322347429No Hit
TCCTTATACCATATAGAAAAATCAACCCAAGATGGATTAAAGACTTAAAT8390.34542812089572517No Hit
CTTCTACTGTTCCTGTCTTTAACCCTTATTGGAAAACACCCTCTTTTCCT6640.2733781552738516No Hit
GCCTAATCCAATGTCCTGGAGCCTTTTCCCATTTTAAAAATTAGTTTCAT6430.2647321593992268No Hit
GCCTAATCAGTTATCTTAGCACCATTTATTAAATAGAGAATCCTTTCCCC6140.2527924508104592No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5840.24044102813242374No Hit
GCCTAATCCAATGTTTTGAAGATTTTCCCCAGTGTTTTCTTTTAGTGGTT5310.21862018140122774No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4960.20421018827685303No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4910.20215161783051377No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4910.20215161783051377No Hit
GCCTAATCCAGTTTTATTCCAGTAGTTTCATAGTTTCAGGTATTAGATTT4790.1972110487592996No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT4370.17991905701004993No Hit
GATTAGGATGGTATAATATTTCCTAGGTTATCTTCCAGGGGCTTTATAGC4360.17950734292078208No Hit
GGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGG4360.17950734292078208No Hit
GTGCTGGTGATAACTGGCTAGCCATATGCCAAAGATTGAACTAGATCCCT4320.1778604865637107No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4280.1762136302066393No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4180.17209648931396082No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG4110.16921449068908587No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG3970.163450493439336No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3880.15974506663592533No Hit
GTTTTTAATAAGTAGTGCTGGGAAAACTGGATATCCATATGCAGAAGAAT3860.15892163845738966No Hit
GGTACAAAAACAGACACATAAACCATTGGAACAGAATAGAGAGCCCAGAA3820.15727478210031826No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3540.1457467876008185No Hit
GGTGATAACTGGCTAGCCATATGCCAAAGATTGAACTAGATCCCTTCCTT3530.14533507351155064No Hit
GCCTGAGGATGCGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT3340.1375125058154615No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA3340.1375125058154615No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCC3280.1350422212798544No Hit
ACATAATAGAGAACCCAGAAATAAGTCCACACACCTACAACCATCTGATC3260.13421879310131873No Hit
TCCTCAGGCCGTGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG3190.13133679447644378No Hit
GGGTTTGTATAGTTTTGAGTTTTACATTTAAGTCTTTAATCCATCTTCGG3120.12845479585156883No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTTATTGGAAAACACCCTCTTTTC3040.12516108313742605No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC2920.12022051406621187No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA2920.12022051406621187No Hit
TCTTTACACTGCTCCTATAAATTGAATTATTATGTACTCTTTTGAATCTA2890.1189853717984083No Hit
GACCAATGGAACAAAATACAGAACCCAGAAATAAAGCCCTGGACCTACAG2890.1189853717984083No Hit
GTTTGGTGGAAGGTTGTGGAGTTCCACTGCACGGCCTGAGGATGCGTGTT2830.11651508726280121No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC2810.11569165908426551No Hit
GCCTAATCAGTTTTCCCAGCATCATTTATTAAATAGGGAATCCTTTCCCC2790.11486823090572983No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA2700.1111628041023192No Hit
GTCATATGATTAGGATGGTATAATATTTCCTAGGTTATCTTCCAGGGGCT2650.10910423365597993No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT2620.10786909138817638No Hit
TCCCTTCCTTACACCTTATACAACAATTAATTCAAGATGGATTAAAGACT2550.10498709276330145No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT2550.10498709276330145No Hit
CTATATCACAATGTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGT2430.10004652369208727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)