FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484020
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70761.4619230610305358No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTAGA17800.36775339861989176No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTGGGTCCCCT10200.21073509359117393No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT9430.1948266600553696No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8220.16982769307053427No Hit
CCCTTATCTAATGGCAAATATTTGGTAACACCTGGATAAAACCTAGCAGG8020.16569563241188381No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACACCTGGATAAAAC7970.1646626172472212No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7950.16424941118135614No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7710.15929093839097558No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7400.15288624437006737No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7340.1516466261724722No Hit
CTCCAAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA7230.14937399281021446No Hit
CATTTAAACACTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT7130.1473079624808892No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7130.1473079624808892No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG6830.1411098714929135No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC6740.1392504441965208No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6530.13491178050493782No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6460.13346555927441014No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC6450.13325895624147763No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTCTGT6270.1295401016486922No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6170.12747407131936697No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCAAAACC6130.12664765918763687No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACACC6120.12644105615470436No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT6090.12582124705590678No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA5870.12127598033139127No Hit
GTGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTAGATGTTCTGGAT5700.11776372877153837No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACACCTGGATAAAA5630.1163175075410107No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5460.1128052559811578No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5450.11259865294822528No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC5110.10557414982851948No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5020.10371472253212677No Hit
GTATCAAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGA5010.10350811949919425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)