FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464703
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA54171.1656907745377154No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21800.4691168337626398No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT21030.45254711073524384No Hit
AGATAAGGGTATCAAACCTTATTATCCAGAACATCTAGTTAATCATTACT17890.38497707137677184No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG15420.3318248429642158No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14100.30341960348867986No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT13960.3004069265746079No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT13070.28125490904943584No Hit
GGATAATAAGGTTTGATACCCTTATCTAATGGCAAATATTTGGTAACATT12160.2616725091079679No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12110.26059655306722784No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11950.25715349373685986No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC11190.24079896191761188No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10760.23154573996724792No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA10440.2246596213065119No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC10400.22379885647391992No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATCAAACCTTAT10390.2235836652657719No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT10330.22229251801688393No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT9970.21454563452355588No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG9840.21174814881763188No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9580.20615317740578393No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG8670.1865707774643159No Hit
ATTTGTAGGTCCACTCACAGTCAATGAAAAACGAAGACTGCAATTAATTA8500.18291252692579993No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT8300.1786087027628399No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8160.1755960258487679No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8020.17258334893469593No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA8010.17236815772654793No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT7910.17021624564506793No Hit
ACCCATAACAGCCAATAACATAGCCCATGAAATTTAAAGAGTAACCCCAT7760.16698837752284793No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCTTGTATT7280.15665919953174393No Hit
GCCAATAACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTT7040.15149461053619193No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGT7040.15149461053619193No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGACTGT6980.15020346328730394No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC6770.14568444791619595No Hit
GTTCTGGATAATAAGGTTTGATACCCTTATCTAATGGCAAATATTTGGTA6670.14353253583471592No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6620.14245657979397594No Hit
TTCTACGACCGTTTCTCTTCCAAAAGTGAGACAAGAAGTGTGAAACCACA6600.14202619737767994No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6580.14159581496138396No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC6370.13707679959027594No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT6300.13557046113323992No Hit
ATGTTATTGGCTGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA6210.13363374025990796No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6130.13191221059472397No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTTTACAAG6110.13148182817842793No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6090.13105144576213193No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA6080.13083625455398395No Hit
GTCTATAAGCTGGTGGAGTGCGAATCCACACTCCGAAAGACACCAAATAT6050.13019068092953995No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTTTCA6030.12976029851324394No Hit
TCCCGATACAGAGCTGAGGCGGTATCAAGAAGATCCCGTACTGAAGGAAA6020.12954510730509594No Hit
TAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTA6020.12954510730509594No Hit
AATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTAG6010.12932991609694794No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5940.12782357763991195No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT5890.12674762159917197No Hit
GTTATTGGCTGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT5760.12395013589324795No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5760.12395013589324795No Hit
CCACCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA5660.12179822381176794No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT5570.11986150293843593No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5420.11663363481621596No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5390.11598806119177195No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5380.11577286998362396No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG5370.11555767877547596No Hit
CACTAATATGGGCTTAAAGTTCAGGCAACTATTGTGGTTTCACACTTCTT5360.11534248756732794No Hit
CTAATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTA5290.11383614911029194No Hit
ATTAATTGCAGTCTTCGTTTTTCATTGACTGTGAGTGGACCTACAAATTG5250.11297538427769994No Hit
TCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCTTAAAGTT5220.11232981065325595No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCAAGAAGATCCCGTA5220.11232981065325595No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5210.11211461944510795No Hit
GTTTGATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC5150.11082347219621995No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATCAAAC5090.10953232494733195No Hit
CAATAACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTG5080.10931713373918395No Hit
CTCACAGTCAATGAAAAACGAAGACTGCAATTAATTATGCCTGCTAGGTT5030.10824117769844396No Hit
CTCTTTTCCTAATATACATTTACACCAAGACATCATCAAAAAATGTGAAC4930.10608926561696395No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTTTCATTGAC4900.10544369199251996No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA4850.10436773595177995No Hit
TTCTAAAACAGTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGAC4730.10178544145400395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)