FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464703
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA51631.1110322076681234No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21710.46718011288930783No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT19190.4129519284360118No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG16350.3518376253219799No Hit
AGATAAGGGTATCAAACCTTATTATCCAGAACATCTAGTTAATCATTACT14640.31503992872867187No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14570.31353359027163585No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT12570.2704953486420359No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT12480.2685586277687039No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT11660.2509129487005679No Hit
GGATAATAAGGTTTGATACCCTTATCTAATGGCAAATATTTGGTAACATT11520.2479002717864959No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10010.21540639935614792No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9790.21067219277689192No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9690.2085202806954119No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC9260.1992670587450479No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATCAAACCTTAT9030.1943176609576439No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8670.1865707774643159No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8600.18506443900727992No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT8280.17817832034654393No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT8140.17516564343247193No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG8120.17473526101617592No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8000.17215296651839992No Hit
ATTTGTAGGTCCACTCACAGTCAATGAAAAACGAAGACTGCAATTAATTA8000.17215296651839992No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA7990.17193777531025192No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7380.15881111161322395No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCTTGTATT7370.15859592040507592No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT7160.15407690503396793No Hit
GTTCTGGATAATAAGGTTTGATACCCTTATCTAATGGCAAATATTTGGTA7040.15149461053619193No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGACTGT7000.15063384570359994No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6790.14611483033249195No Hit
GTTTGATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC6770.14568444791619595No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG6680.14374772704286393No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC6490.13965909408805194No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT6400.13772237321471995No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGT6210.13363374025990796No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC6140.13212740180287194No Hit
ATGTTATTGGCTGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA5920.12739319522361595No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTTTCA5900.12696281280731994No Hit
TTCTACGACCGTTTCTCTTCCAAAAGTGAGACAAGAAGTGTGAAACCACA5880.12653243039102394No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA5790.12459570951769196No Hit
GTCTATAAGCTGGTGGAGTGCGAATCCACACTCCGAAAGACACCAAATAT5660.12179822381176794No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5620.12093745897917595No Hit
ACCCATAACAGCCAATAACATAGCCCATGAAATTTAAAGAGTAACCCCAT5600.12050707656287994No Hit
TAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTA5400.11620325239991994No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5360.11534248756732794No Hit
GTTATTGGCTGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT5150.11082347219621995No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5110.10996270736362795No Hit
AATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTAG5090.10953232494733195No Hit
ATTAATTGCAGTCTTCGTTTTTCATTGACTGTGAGTGGACCTACAAATTG5070.10910194253103596No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATCAAAC4910.10565888320066795No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTTTCATTGAC4870.10479811836807595No Hit
GCCAATAACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTT4850.10436773595177995No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG4850.10436773595177995No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4830.10393735353548396No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4830.10393735353548396No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4810.10350697111918795No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTTTACAAG4790.10307658870289196No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4750.10221582387029997No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA4740.10200063266215195No Hit
CCTACAAATTGTTCACATTTTTTGATGATGTCTTGGTGTAAATGTATATT4660.10027910299696796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)