FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381334
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA27910.7319043148525964No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT15460.4054188716453293No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14490.37998185317857835No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC12980.3403840203076568No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12130.3180938494862771No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAAC12050.3159959510560296No Hit
CCTAATACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC11900.3120623914993156No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10450.27403798245107963No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10320.2706288975019274No Hit
CCTATTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC10000.26223730378093746No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8880.23286672575747244No Hit
GGGACTCACCCCACCGCACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTC8510.2231639455175778No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA8430.22106604708733027No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC8360.2192303859608637No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8060.2113632668474356No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7890.20690523268315966No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7580.19877587626595059No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG7550.19798916435460776No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC7450.1953667913167984No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC7320.1919577063676462No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7250.19012204524117965No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7230.18959757063361776No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7140.18723743489958933No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7100.18618848568446558No Hit
GTGAAAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAA6870.18015702769750402No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC6740.17674794274835184No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6630.17386333240676152No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6580.17255214588785683No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6550.171765433976514No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6370.16704516250845713No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6300.1652095013819906No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG6260.16416055216686684No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5970.15655567035721965No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5970.15655567035721965No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5920.15524448383831496No Hit
CCTTTTACACAATGTGGTTATCCTGCCTTAATGCCCTTGTATGCATGTAT5860.15367106001562936No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5790.15183539888916278No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5770.15131092428160092No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT5580.14632841550976308No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5340.14003472021902058No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTATTACAAAGACC5280.13846129639633498No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT5260.1379368217887731No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5240.13741234718121123No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5200.13636339796608749No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5170.13557668605474466No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT5170.13557668605474466No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5150.1350522114471828No Hit
GGGAAATACAGGCCTTTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT5130.1345277368396209No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5130.1345277368396209No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC5070.13295431301693528No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTG5050.1324298384093734No Hit
CTCACAGTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT5050.1324298384093734No Hit
CTCCTATTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAG5030.13190536380181153No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGATCACCATATT4990.13085641458668779No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG4970.13033193997912593No Hit
GAATACATGCATACAAGGGCATTAAGGCAGGATAACCACATTGTGTAAAA4950.12980746537156404No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4910.1287585161564403No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4860.1274473296375356No Hit
GGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTC4810.12613614311863092No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4750.12456271929594528No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4740.12430048199216434No Hit
CATGCATACAAGGGCATTAAGGCAGGATAACCACATTGTGTAAAAGGAGC4740.12430048199216434No Hit
GCTTTATTCTTCTTCTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT4690.12298929547325965No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4680.12272705816947874No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA4630.12141587165057403No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4600.12062915973923122No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTGTTCCTGTC4490.11774454939764091No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4470.11722007479007904No Hit
GTATCAAACCTTATTATCCAGAACATCTGGTTAATCATTACTTCCAAACC4460.11695783748629811No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4450.11669560018251715No Hit
GTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTACCC4440.11643336287873622No Hit
CTTCTAGATACCGCCTCAGCTTTATATCGGGAAGCCTTAGAGTCTCCTGA4400.11538441366361248No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4370.11459770175226967No Hit
GATTATGCCTGCTAGGTTTTACCCAAATGTTACCAAATATTTGCCATTGG4310.11302427792958404No Hit
GAATACTAACAGTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4300.11276204062580311No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT4300.11276204062580311No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4300.11276204062580311No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAA4300.11276204062580311No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4290.11249980332202215No Hit
GCTTTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA4290.11249980332202215No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4270.11197532871446028No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4260.11171309141067935No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4250.11145085410689841No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4210.11040190489177468No Hit
ATATAAAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT4190.10987743028421279No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4140.10856624376530809No Hit
GCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCC4090.10725505724640341No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA4060.10646834533506061No Hit
GAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTACCCAAATGTTA4020.10541939611993685No Hit
GTGAATCCCAGCCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCCA3980.10437044690481309No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC3970.10410820960103218No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3960.10384597229725122No Hit
GCCTATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC3960.10384597229725122No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA3960.10384597229725122No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG3930.10305926038590842No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT3880.10174807386700373No Hit
GTCATAGGCCATCAGCGCATGCGTGGAACCTTTCTGGCTCCTCTGCCGAT3880.10174807386700373No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC3880.10174807386700373No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAATTAATGA3870.10148583656322278No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGATCACCATA3870.10148583656322278No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT3860.10122359925944185No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC3860.10122359925944185No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC3860.10122359925944185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)