FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407415
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA35730.8769927469533522No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30030.737086263392364No Hit
ATCCCAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCCTAT27490.6747419707178184No Hit
GTGCTGGTGTGATGGTGTCCTACTTTCTAGGTGGTGTCAAGAACTTTCTA24940.6121522280721132No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT24420.5993888295718125No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCCTAT24420.5993888295718125No Hit
GTGATAACATTAGCCAGAATATCCGTTTTTTGCACTCATTCTCTGAAGTC12630.31000331357461064No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT12120.29748536504546963No Hit
TGTTATCACTGTGTGTAATGAATTGTCCTGTTTCTCTGAACCAAGAGTCA11650.2859492164009671No Hit
TCATTACACACAGTGATAACATTAGCCAGAATATCCGTTTTTTGCACTCA9690.2378410220536799No Hit
ATATTGATGATGTGGTACTGGGGGCCAAATCTGTACAGCATCTTGAGTCC9490.23293202263048735No Hit
CTTGTATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT9400.23072297289005067No Hit
GTGTGATGGTGTCCTACTTTCTAGGTGGTGTCAAGAACTTTCTAGGTGGT9230.226550323380337No Hit
CTCTAAGCCGAGGTTTTAGCTCCTAAAAGCCCCCCTCCTCCCCCACTGCA8970.22016862413018665No Hit
GGATAAGGGTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACT8970.22016862413018665No Hit
CATCTAGCCTTCTTCACATTTCCTCATAGAAACTGGACTTCAGAGAATGA8860.21746867444743076No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8760.21501417473583448No Hit
CTGTAGATCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGCTAT8380.20568707583176857No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8180.200778076408576No Hit
GTGTAATGAATTGTCCTGTTTCTCTGAACCAAGAGTCATGTGTCTTCTGC7830.19218732741798902No Hit
GTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7290.17893302897536909No Hit
ACACCAATGTGGGCCTAAAATTCAGGCAACTATTGTGGTTTCACATTTCT7220.1772148791772517No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7140.17525127940797466No Hit
GCTAATAAGTTAGCCTATCACAGTTTCATGGATGCTGGCAGAAGACACAT6620.162487880907674No Hit
CTAATAAGTTAGCCTATCACAGTTTCATGGATGCTGGCAGAAGACACATG6570.16126063105187585No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6520.1600333811960777No Hit
ATCTAGCCTTCTTCACATTTCCTCATAGAAACTGGACTTCAGAGAATGAG6330.15536983174404476No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6310.15487893180172552No Hit
GTCAAGAACTTTCTAGGTGGTGTCAAGCTCTGAGATTCTTACCTGACATG6240.1531607820036081No Hit
GAAAAGAAGACTGAAATTGATTATGCCTGCTAGATTTTATCCCAAGGTTA6170.15144263220549073No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC6090.1494790324362137No Hit
GTGGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGCTATGT6050.14849723255157518No Hit
CCTCCCCCACTGCATGTGCAGGCATCATCTAGCCTTCTTCACATTTCCTC5930.14555183289765963No Hit
ATTCATTACACACAGTGATAACATTAGCCAGAATATCCGTTTTTTGCACT5910.14506093295534037No Hit
CTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5850.1435882331283826No Hit
ATGTTATCACTGTGTGTAATGAATTGTCCTGTTTCTCTGAACCAAGAGTC5690.13966103358982854No Hit
GGCTAATGTTATCACTGTGTGTAATGAATTGTCCTGTTTCTCTGAACCAA5470.13426113422431674No Hit
ACTTTCTAGGTGGTGTCAAGAACTTTCTAGGTGGTGTCAAGCTCTGAGAT5390.1322975344550397No Hit
CTTTCTAGGTGGTGTCAAGCTCTGAGATTCTTACCTGACATGCAAGCTAA5200.12763398500300677No Hit
CTCTTGTATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5150.12640673514720863No Hit
GGCATCATCTAGCCTTCTTCACATTTCCTCATAGAAACTGGACTTCAGAG5100.12517948529141049No Hit
CTTCTCTTCTCATTAACTGTGAGTGGGCCTACAAATTGTTCACATTTTTT5070.1244431353779316No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5050.12395223543561233No Hit
GCCTATCACAGTTTCATGGATGCTGGCAGAAGACACATGACTCTTGGTTC4970.1219886356663353No Hit
TATAAAGGCTTTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCGAA4870.11953413595473902No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT4800.11781598615662162No Hit
CTGCATGTGCAGGCATCATCTAGCCTTCTTCACATTTCCTCATAGAAACT4790.11757053618546201No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4640.11388878661806756No Hit
AAGTTAGCCTATCACAGTTTCATGGATGCTGGCAGAAGACACATGACTCT4610.11315243670458869No Hit
GAGCCACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCT4600.11290698673342907No Hit
CATTAGTGTTGACATAACCGACTACTAGGTCCCTGGATGCTGGATCTTCT4580.11241608679110979No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4550.11167973687763091No Hit
CAAGATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGAT4540.1114342869064713No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC4500.11045248702183277No Hit
CCCCCACTGCATGTGCAGGCATCATCTAGCCTTCTTCACATTTCCTCATA4500.11045248702183277No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4430.10873433722371538No Hit
GGATAATAGGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT4430.10873433722371538No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTGGGATAAAAT4330.10627983751211909No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA4320.10603438754095947No Hit
ACATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4270.10480713768516133No Hit
GTAATGAATTGTCCTGTTTCTCTGAACCAAGAGTCATGTGTCTTCTGCCA4260.10456168771400169No Hit
TGATGATGGTGTCCAGAGTAAAAAAAGGTTTGTCAGTTTTCCATTATAAA4230.1038253378005228No Hit
GTGTCAAGCTCTGAGATTCTTACCTGACATGCAAGCTAATAAGTTAGCCT4220.10357988782936317No Hit
AATCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4180.10259808794472466No Hit
GGGTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACTTCCAAA4130.10137083808892654No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT4110.10087993814660726No Hit
CATTTAAACCCCAACAAAACAAAACGATGGGGTTACTCTTTACATTTCAT4080.10014358823312837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)