FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422364
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35540.8414542906118893No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA23000.5445539866087072No Hit
AATATACACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG11430.27061965508424013No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10420.2467066321940317No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT10230.24220814273943803No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9930.2351052646532375No Hit
CATTTAAACCCTGACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT9700.2296597247871504No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9650.22847591177278365No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9390.22232008409807652No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8400.19888058641361478No Hit
CACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8380.19840706120786808No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8100.19177770832741425No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC7390.17496756352340634No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7300.17283670009754618No Hit
GGATAATAGGGTTTAATTCCCTTATCTAATGGCAAATATTTGGTGACCTT7260.1718896496860528No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7200.17046907406881268No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7140.16904849845157258No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7110.16833821064295254No Hit
ATCCAAAGGTCACCAAATATTTGCCATTAGATAAGGGAATTAAACCCTAT6760.16005151954238525No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA6170.14608252597285754No Hit
CCTTTGGATAGAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA5890.1394531730924037No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCAGCTACCAATCGCCAATC5500.13021943158034302No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5480.12974590637459632No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCATA5430.12856209336022956No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5030.11909158924529553No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5020.11885482664242218No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4820.11411957458495517No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG4740.11222547376196834No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGCGGGCGTTC4730.111988711159095No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG4730.111988711159095No Hit
CACTAATATGGGCCTGAAGTTCAGGCAAATATTATGGTTTCACATTTCTT4720.11175194855622166No Hit
CTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4610.10914755992461479No Hit
CAATAGGCCTGTTGACAGGAAGTTTTCGAAAACAGTCTTTGATTTTCTTT4610.10914755992461479No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4600.10891079732174144No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACA4560.10796374691024804No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4560.10796374691024804No Hit
AGATAAGGGAATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACT4490.10630640869013458No Hit
GGATAGAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC4480.10606964608726123No Hit
GTCTTGGTGTAAGTGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT4420.10464907047002112No Hit
CTCACAGTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTT4410.10441230786714777No Hit
GCAATGACCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAGTAA4400.10417554526427442No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4350.10299173224990767No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4340.10275496964703432No Hit
GTGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGACAGGTA4310.10204468183841425No Hit
ATGTAAGACCTTGGGCAAGAATTGGCGGGCGTTCACGGTGGTCTCCATGC4290.10157115663266755No Hit
GTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTCTATCC4290.10157115663266755No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC4260.1008608688240475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)