FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411351
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTGTGGTTTGGAAGTAATGATTAACTAGA24960.6067810701809404No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13430.32648516716867104No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10370.25209614173783457No Hit
GTGTAAATAATGTGTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9970.24237208612596056No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC9800.2382393624909141No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC9070.22049296099924393No Hit
CATTTACACCAAGACATTATCCAACGATGTGAACAATTTGTAGGCCCACT9030.21952055543805654No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTGTGGT8760.21295681790004156No Hit
CTCTATGACCGTTTCTTTTCCAAAAGTGAGACAAGAAATGTGAAACCATA8730.212227513729151No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTGTGGTTT8610.2093102970455888No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8540.20760858731351087No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8410.2044482692396518No Hit
CATTTAAACCCTCACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT7920.19253630111510608No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTTTTATGGTTTCACATTTCTT7710.1874311719188722No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7630.18548636079649738No Hit
CACTCACAGTCAATGAAAAACGAAGACTGCAATTAATTATGCCTGCTAGG7190.17478989962343594No Hit
CACTTACAGTCAATGAAAAACGAAGACTGCAATTAATTATGCCTGCTAGG6860.16676755374363986No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG6730.1636072356697808No Hit
CTTTTACACAATGTGGTTATCCTGCCTTAATGCCCTTGTATGCCTGTATT6570.15971761342503116No Hit
GGATAATACGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT6130.14902115225196974No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6060.14731944251989176No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTGTGGTTTGGA6050.14707634112959492No Hit
GTACAGCACCTTGAGTCCCTTTTTACTGCTGTTACCAATTTTCTTTTGTC5970.14513153000722012No Hit
ACACCAAATACTCTATGACCGTTTCTTTTCCAAAAGTGAGACAAGAAATG5830.1417281105430642No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5660.13759538690801773No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTGTGGTTTGGAAGT5500.1337057646632681No Hit
GTGTGTAAATAATGTGTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5480.1332195618826744No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5370.13054544658940903No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5330.12957304102822165No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT5150.12519721600287834No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT5070.12325240488050351No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5010.12179379653872241No Hit
CCCTCACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCATGGGCTATG4950.12033518819694129No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC4820.11717487012308224No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4770.11595936317159798No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCA4740.11523005900070742No Hit
AGACTGTACAGCACCTTGAGTCCCTTTTTACTGCTGTTACCAATTTTCTT4720.11474385622011372No Hit
AATATACATTTACACCAAGACATTATCCAACGATGTGAACAATTTGTAGG4670.11352834926862947No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT4640.1127990450977389No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4510.10963872702387985No Hit
GTATGATGTGATCTTGTGGCAAGGACCCATAACATCCAATGACATAGCCC4460.1084232200723956No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4430.10769391590150504No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTTTCATTGAC4400.10696461173061449No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4370.10623530755972395No Hit
ATGTCATTGGATGTTATGGGTCCTTGCCACAAGATCACATCATACAGAAA4220.10258878670527116No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTTTCA4160.10113017836349007No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4150.10088707697319321No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC4120.10015777280230266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)