FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468205
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG53031.1326235302912186No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT38590.8242116167063572No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35900.7667581508100084No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAAAAAG35190.751593853119894No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT25950.5542444014908No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT23700.50618852852917No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAAAA18160.3878642902147564No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC16610.3547591332856334No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT16040.3425849788020205No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA15690.3351096207857669No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14480.3092662402152903No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14080.3007229739110005No Hit
CTCTCACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13140.28064629809591957No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT12710.27146228681880796No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT12290.26249185719930374No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11890.2539485908950139No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC11400.24348308967225896No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC10920.2332311701071112No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10270.2193483623626403No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC10210.21806687241699682No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTT10090.2155038925257099No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9530.2035433196997042No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC9330.1992716865475593No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA9110.1945728900801999No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8790.18773827703676807No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT8660.1849617154878739No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8570.1830394805694087No Hit
GTCTTATATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT8500.18154440896615798No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA8330.1779135207868348No Hit
GAATATGGTGACCCACAAAATGAAGCACTTCGTGTAGTTTCTCTCTTATA8330.1779135207868348No Hit
CTCAAGAACAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8100.1730011426618682No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7880.16830234619450882No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG7830.16723443790647258No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTGTCTCTTCCAAAAGTAAGA7760.1657393663032219No Hit
ATATTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAG7440.15890475325979006No Hit
GTCTATTTACAGGCAATTTCCGAAAACATTGCTTGAGTTTTAGTACAATA7340.1567689366837176No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA7290.15570102839568137No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7230.15441953845003792No Hit
CTCACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT7210.15399237513482342No Hit
CTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7170.15313804850439444No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7140.1524973035315727No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT7060.15078865027071475No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7060.15078865027071475No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7050.15057506861310752No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCCTTTC7020.14993432364028578No Hit
CTTTAACCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6960.1486528336946423No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG6880.14694418043378435No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6870.14673059877617708No Hit
AGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA6790.14502194551531913No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG6660.14224538396642494No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGGAAATTGCCTGTAAATAGA6630.14160463899360323No Hit
GATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT6450.1377601691566728No Hit
GAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAACCT6370.13605151589581485No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6350.13562435258060038No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA6340.13541077092299314No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTCCGAAAAC6330.13519718926538588No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6260.13370211766213516No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6150.1313527194284555No Hit
CAATAGGTCTATTTACAGGCAATTTCCGAAAACATTGCTTGAGTTTTAGT6090.13007122948281202No Hit
AGAATATGGTGACCCACAAAATGAAGCACTTCGTGTAGTTTCTCTCTTAT6050.12921690285238305No Hit
GGTCTATTTACAGGCAATTTCCGAAAACATTGCTTGAGTTTTAGTACAAT5990.12793541290673957No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5970.12750824959152507No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5840.12473168804263089No Hit
CATTATTAATAGGTGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAA5840.12473168804263089No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAATTGCCTGTAA5830.12451810638502364No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC5770.12323661643938019No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5730.12238228980895122No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCT5710.12195512649373672No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5680.12131438152091498No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5650.12067363654809325No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC5580.11917856494484252No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5440.11618842173834111No Hit
CAATATGTGGGCCCTCTCACAGTTAATGAAAAAAGGAGATTAAAATTAAT5410.11554767676551939No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC5410.11554767676551939No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5380.11490693179269763No Hit
GTCTTTAACCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACA5300.11319827853183968No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC5290.11298469687423243No Hit
GTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATG5280.11277111521662521No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC5250.11213037024380346No Hit
CAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTTTAT5250.11213037024380346No Hit
ACCCAGCATCCAGGGAGTTAGTAGTCAGCTATGTCAATGTTAATATGGGC5240.11191678858619622No Hit
CTATATAAGAGAGAAACTACACGAAGTGCTTCATTTTGTGGGTCACCATA5170.11042171698294549No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG5130.10956739035251652No Hit
CATTTACAGGAGGACATTATTAATAGGTGTCAACAATATGTGGGCCCTCT5100.10892664537969479No Hit
GCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT5030.10743157377644408No Hit
ACAATATGTGGGCCCTCTCACAGTTAATGAAAAAAGGAGATTAAAATTAA5010.10700441046122959No Hit
ATGAAGCACTTCGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCAC4980.10636366548840785No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4970.1061500838308006No Hit
CCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGC4960.10593650217319336No Hit
GGACATTATTAATAGGTGTCAACAATATGTGGGCCCTCTCACAGTTAATG4940.10550933885797888No Hit
ATGTCAATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCAC4870.10401426725472818No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACATAC4860.10380068559712093No Hit
ATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAG4860.10380068559712093No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4840.10337352228190642No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4830.1031599406242992No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAA4810.1027327773090847No Hit
AAATGAAGCACTTCGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCC4690.10016979741779776No Hit
ATTTACAGGAGGACATTATTAATAGGTGTCAACAATATGTGGGCCCTCTC4690.10016979741779776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)