FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430490
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39200.9105902576134173No Hit
ATATAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC12070.28037817370902923No Hit
GTATGTAAATAATGCCTTGTTTTGAAGTAATGATTAACTGCATGTTCAGG11740.27271249041789586No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9960.2313642593323887No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTTGTTT9260.21510371901786338No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8510.1976817115380148No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7940.18444098585332994No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7710.17909823689284304No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7020.16306999001138237No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6990.16237310971218843No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGTCCGACCT6780.15749494761783084No Hit
CTATTAATAATGTCCTCCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTG6650.15447513298799043No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6600.1533136658226672No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6590.15308137238960254No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6040.14030523357104696No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6040.14030523357104696No Hit
CCCTAGCAGCCATGGGAATGAGGTGTATTTCCGAGAGAGGACAACTGAGT5990.1391437664057237No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTTGT5940.13798229924040048No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG5790.13449789774443077No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5740.13333643057910752No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5700.13240725684684895No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5690.1321749634137843No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5640.13101349624846106No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5640.13101349624846106No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5480.1272968013194267No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5280.12265093265813375No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA5280.12265093265813375No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5150.11963111802829333No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5090.11823735742990545No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA4970.11544983623312968No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTTT4900.11382378220167716No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4900.11382378220167716No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4860.11289460846941857No Hit
ATGTTAATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCAC4820.11196543473715999No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4800.11150084787103069No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGACGTTGACATTGCTGAGAGTCCAA4790.11126855443796604No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4770.11080396757183675No Hit
CATTAACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTA4690.10894562010731956No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4560.10592580547747915No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4380.10174452368231549No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4370.10151223024925085No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4370.10151223024925085No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4360.10127993681618622No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4340.1008153499500569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)