FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631662
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48210.7632246359603712No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGCAAATAATGCCTTGT29740.47082142031656166No Hit
TCCAAAGAGTATGCAAATAATGCCTTGTTTGGAAGTAATGATTAACTACA21320.33752228248651966No Hit
CTGTTTACTCAGAAAGGCCTTATAAGTTGGCGAGAAAGTAAAAGCCTGTT16540.26184890020295665No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAAACAAGGCATTATT13210.20913083262884263No Hit
CTTTAATCCTGAATGGCAAACTCCTTCTTTTCCAGACATTCATTTGCAGG12890.2040648321412401No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGCAAATAATGCCTTGTTTGGA12020.1902916433155707No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACGGGTCTCTATTC11240.17794326712703945No Hit
GTGTTGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTATGCAAA10990.17398545424609996No Hit
CCTGTTTTCATTTACTGTAAGGGGTCCCACAAATTGCTTACATCTATCAA10980.1738271417308624No Hit
GTATGCAAATAATGCCTTGTTTGGAAGTAATGATTAACTACATGCTCTGG9820.15546288996330315No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAAC9520.15071351450617576No Hit
GTATACATGCATATAAAGGCTTTAAAGCAGGATATCCACATTGGGTAAAA9380.14849713929284966No Hit
GTCTTCAATCCTGAATGGCAAACTCCTTCTTTTCCAGACATTCATTTGCA9360.1481805142623745No Hit
GTCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCAGACATTCATTTGCA9200.14564751401857323No Hit
GTCTTATATAAGAGGACTCTTGGACTTTCAACAATGTCAACGACCGACCT8790.13915670089383247No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACGGGTCTCTATTC8770.1388400758633573No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATATCCTCCTGC8640.13678201316526878No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGTTGAAAGTCCAA8440.13361576286051718No Hit
GTTTGGAAGTAATGATTAACTACATGCTCTGGATAATAAGGTTTGATCCC8370.13250757525385412No Hit
GGAACATATTGTACAAAAAATCAAACTATGTTTTAGAAAACTTCCTGTAA8330.1318743251929038No Hit
CTGTTTACTCAGAAAGGCCTTATAAGTTTGCGAGAAAGTAAAAGCCTGTT8270.13092445010147832No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8170.12934132494910253No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG7960.12601676212911336No Hit
GCGTCACCAGTATCTGACCCTTTACCCCGTTGCTCGGCAACGGCCTGGTC7810.12364207440054965No Hit
GTTTACAGGAAGTTTTCTAAAACATAGTTTGATTTTTTGTACAATATGTT7780.12316713685483693No Hit
GTATCGGGAAGCCTTAGAAAGGAACATATTGTACAAAAAATCAAACTATG7580.12000088655008533No Hit
GCTTTAAAGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT7460.11810113636723438No Hit
CTTTTACCCAATGTGGATATCCTGCTTTAAAGCCTTTATATGCATGTATA7310.11572644863867068No Hit
GTGGATATCCTGCTTTAAAGCCTTTATATGCATGTATACAAGCAAAACAG7250.1147765735472452No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCT7130.11287682336439425No Hit
CTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATAGTTTG7010.11097707318154329No Hit
ATATAAGACCTTGGGCAGGCTCCGGTGGGCGTTCACGGTGGTTTCCATGC6840.10828576042250443No Hit
TCCCGATACAGAGCAGCAGCGGTATCTAGGAGGTCTCGCACCGACGGAAA6800.10765251036155413No Hit
CTTCAATCCTGAATGGCAAACTCCTTCTTTTCCAGACATTCATTTGCAGG6760.10701926030060381No Hit
GGATATTGTTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATG6580.10416963502632737No Hit
AATGCTACCAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCC6580.10416963502632737No Hit
GAGTATGCAAATAATGCCTTGTTTGGAAGTAATGATTAACTACATGCTCT6560.10385300999585222No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATAGTTTGATTTTTTGT6380.10100338472157579No Hit
GTGCTGGTGGACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6330.10021182214538789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)