FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462172
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT15400.33320928139307443No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC14620.31633244766017843No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14090.30486485550833886No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG13120.28387699817384004No Hit
GTATTCATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAC13080.283011519520871No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC12690.27457310265442303No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12070.26115818353340314No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA11840.25618168127883123No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAC10850.2347610846178479No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT10830.2343283452913634No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10370.2243753407822196No Hit
TATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT10300.22286075313952383No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10160.21983157785413224No Hit
ATTAATAGGTGTGAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG9870.21355685762010682No Hit
TTGTTCACACCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGG9730.21052768233471522No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA9710.2100949430082307No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC9640.20858035536553493No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT9300.20122378681529818No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT9280.20079104748881368No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT9150.19797824186666438No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC8980.194299957591546No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT8940.193434478938577No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG8730.1888907160104896No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCGCATTGTGTAAAT8260.17872134183810356No Hit
ATTCTATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC8160.176557645205681No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT8040.17396120924677394No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGTGGGTCACCATA7980.17266299126732038No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC7660.1657391620435682No Hit
ATGTAAGACCTTGGGCAGGCTCAGATGGGCGTTCACGGTGGTCTCCATGC7490.16206087776844982No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA7290.15773348450360472No Hit
CATGTATACAAGCTAAACAGGCTTTCATTTTCTCGCCAACTTACAAGGCC7230.15643526652415118No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG7200.1557861575344244No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT7080.15318972157551736No Hit
GTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAGAAGATTGA7060.15275698224903284No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATTCATTTAA7040.15232424292254831No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT6920.14972780696364124No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGAGT6840.1479968496577032No Hit
GCCAAAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAGCC6700.14496767437231162No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC6600.14280397773988904No Hit
CCATTTACACAATGCGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6550.14172212942367776No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG6500.14064028110746649No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAATCTCCTGA6380.1380438451485594No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG6220.13458193053668333No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT6140.13285097323074527No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTT6100.13198549457777625No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG5990.1296054282821114No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG5970.12917268895562692No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA5920.12809084063941562No Hit
GGTGACCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG5880.1272253619864466No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5880.1272253619864466No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGTGGGTCACCATATT5860.1267926226599621No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA5780.12506166535402402No Hit
GCTTTGGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5780.12506166535402402No Hit
GGCTCAGATGGGCGTTCACGGTGGTCTCCATGCAACGTGCAGAGGTGAAG5770.12484529569078179No Hit
GTTGACACCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAA5680.12289796872160148No Hit
GTCTAAGGGCAAATATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAA5670.12268159905835922No Hit
ATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGAGTAACTCCA5660.12246522939511698No Hit
ATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT5560.1203015327626944No Hit
CTTTGGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA5510.11921968444648313No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5460.11813783613027184No Hit
GTCAAAGATCTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGA5450.11792146646702958No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5400.11683961815081832No Hit
CTCTTGTACATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC5380.11640687882433379No Hit
AAGTCAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC5360.11597413949784928No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC5240.11337770353894222No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5220.11294496421245771No Hit
GAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA5200.11251222488597319No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT5110.11056489791679289No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG5100.11034852825355063No Hit
TTATTAATAGGTGTGAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG5090.11013215859030838No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5080.10991578892706612No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5060.10948304960058161No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5050.10926667993733934No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4980.10775209229464354No Hit
GGTTTAAGTCCCAATCTGGATTGTTTGAGTTGGCTCCGAATGCAGGGTCC4940.10688661364167452No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4930.10667024397843228No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4920.10645387431519002No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA4900.1060211349887055No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4890.10580476532546326No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT4870.10537202599897873No Hit
GGGTAAAGGTTCATGTACTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGA4840.10472291700925197No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTTTA4810.10407380801952519No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT4810.10407380801952519No Hit
GGGATCTAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGG4800.10385743835628294No Hit
CCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAGTCTTTGA4780.10342469902979844No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTC4720.10212648105034489No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT4700.10169374172386038No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAATGAAAGCCTGTT4640.10039552374440684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)