FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462172
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT15000.3245544948633842No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG13640.2951282206624374No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA13010.28149693187817526No Hit
GTATTCATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAC12650.273707624001454No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC12540.27132755770578915No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12060.2609418138701609No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA11740.2540179846464087No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11230.24298313182105363No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC10880.23541019360757467No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAC10760.23281375764866757No Hit
TATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT10520.22762088573085346No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA10410.22524081943518862No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT10380.22459171044546186No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT9880.21377322728334905No Hit
TTGTTCACACCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGG9450.20446933176393203No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9400.20338748344772076No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT9270.20057467782557142No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC9210.1992764598461179No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT9110.19711276321369536No Hit
ATTAATAGGTGTGAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG9010.19494906658127278No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC8820.1908380429796699No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCGCATTGTGTAAAT8800.1904053036531854No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG8450.18283236543970643No Hit
ATTCTATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC8280.17915408116458809No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGTGGGTCACCATA8080.17482668789974296No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG7850.16985018564517107No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG7810.16898470699220203No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT7800.16876833732895977No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT7770.168119228339233No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG7600.16444094406411466No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA7510.16249361709493435No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATTCATTTAA7370.15946444180954278No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC7320.15838259349333147No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA7300.15794985416684698No Hit
GTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAGAAGATTGA7200.1557861575344244No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG7200.1557861575344244No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC7150.15470430921821315No Hit
GCTTTGGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG7020.15189150359606382No Hit
CTTTGGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA6990.15124239460633704No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGAGT6870.14864595864742997No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAATCTCCTGA6760.14626589235176513No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT6710.14518404403555385No Hit
CATGTATACAAGCTAAACAGGCTTTCATTTTCTCGCCAACTTACAAGGCC6610.1430203474031313No Hit
GTCTAAGGGCAAATATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAA6590.1425876080766468No Hit
CTCTTGTACATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC6430.1391256934647707No Hit
CCATTTACACAATGCGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6380.1380438451485594No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG6340.13717836649559037No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG6320.13674562716910588No Hit
GCCAAAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAGCC6270.13566377885289457No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT6140.13285097323074527No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA6010.13003816760859593No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTC5970.12917268895562692No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5910.12787447097617338No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTT5880.1272253619864466No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGTGGGTCACCATATT5800.12549440468050857No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5740.12419618670105502No Hit
GGTGACCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG5730.12397981703781277No Hit
GTTGACACCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAA5700.12333070804808599No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5650.1222488597318747No Hit
ATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT5620.12159975074214795No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5610.12138338107890569No Hit
ATGTAAGACCTTGGGCAGGCTCAGATGGGCGTTCACGGTGGTCTCCATGC5600.12116701141566344No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC5600.12116701141566344No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT5510.11921968444648313No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5450.11792146646702958No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTTTA5350.11575776983460703No Hit
TTATTAATAGGTGTGAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG5320.11510866084488025No Hit
GTCAAAGATCTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGA5280.11424318219191124No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5200.11251222488597319No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5080.10991578892706612No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5070.10969941926382384No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC5040.10905031027409709No Hit
GGGTAAAGGTTCATGTACTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGA4960.10731935296815903No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4880.10558839566222099No Hit
GAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA4840.10472291700925197No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC4830.1045065473460097No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG4790.10364106869304068No Hit
GGTTTAAGTCCCAATCTGGATTGTTTGAGTTGGCTCCGAATGCAGGGTCC4780.10342469902979844No Hit
ATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGAGTAACTCCA4730.10234285071358716No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG4710.10191011138710264No Hit
AAGTCAACAGTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC4700.10169374172386038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)