FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463977
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG27380.5901154583093559No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT24650.5312763348183207No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT17060.3676906398377506No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC16830.3627334975656121No Hit
TATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT16220.3495862941482013No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA16020.3452757356506896No Hit
GTATACATGCATACAAAGGCATTAATGCAGGATATCCACATTGTGTAAAT15260.328895613360145No Hit
GTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT15130.32609375033676236No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC14340.30906704427159104No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG13730.29591984085418027No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA13450.28988505895766387No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13380.28837636348353474No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA13360.28794530763378356No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG13350.28772977970890795No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT12920.2784620789392578No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT12860.27716891139000427No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT12820.2763067996905019No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC12700.27372046459199484No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA12640.2724272970427414No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG12310.265314875521847No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC12070.26014220532483295No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGAGT12020.25906456570045494No Hit
ATTCTATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC11530.24850369738155126No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC11490.24764158568204886No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG10780.23233910301588226No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG10610.2286751282929973No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA10490.22608879319449024No Hit
TATCCACATTGTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAATTCT10160.21897637167359588No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA9830.21186395015270154No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG9780.21078631052832358No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9630.20755339165518982No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA9390.2023807214581757No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA9260.19957885843479312No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG9200.19828569088553957No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9060.19526829993728137No Hit
GTCTAAGGGCAAATATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAA8610.18556954331787998No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8600.1853540153930044No Hit
CTCCTAGACACCGCGTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA8590.1851384874681288No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG8490.18298320821937294No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC8400.18104345689549267No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG8190.17651737047310537No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTT7940.1711291723512157No Hit
ATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT7920.17069811650146452No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC7890.17005153272683773No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT7860.16940494895221098No Hit
GAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA7860.16940494895221098No Hit
CCATTTACACAATGTGGATATCCTGCATTAATGCCTTTGTATGCATGTAT7660.16509439045469926No Hit
GGATATCCACATTGTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAAT7600.16380122290544574No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC7540.16250805535619223No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA7500.16164594365668988No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG7250.15625774553480024No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT7160.15431799421091993No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA7000.15086954741291056No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG6900.1487142681641547No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6840.14742110061490116No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT6710.14461923759151854No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCA6650.14332607004226502No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC6600.1422484304178871No Hit
GCTTTATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTT6520.1405242070188824No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT6510.14030867909400682No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT6420.13836892777012655No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC6210.13384284134773922No Hit
CATGCATACAAAGGCATTAATGCAGGATATCCACATTGTGTAAATGGAGC6190.13341178549798804No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTC6190.13341178549798804No Hit
GTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT6180.13319625757311246No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6040.13017886662485426No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT5970.12867017115072515No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT5950.12823911530097398No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5940.1280235873760984No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGA5900.12716147567659603No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5840.12586830812734254No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG5790.12479066850296458No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG5780.12457514057808901No Hit
ATGTGGATATCCTGCATTAATGCCTTTGTATGCATGTATACAAGCTAAAC5770.12435961265321342No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCTTATAGACCACCAAATGCC5740.12371302887858665No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG5690.12263538925420872No Hit
ATTTACAAGAGGACATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTC5680.12241986132933314No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5660.12198880547958198No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTACAG5650.12177327755470636No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5630.12134222170495519No Hit
CTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATTCTAT5630.12134222170495519No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT5510.11875588660644816No Hit
TCCCTAAACTTCATGGGTTACATAATTGGAAGTTGGGGAACTTTGCCACA5470.11789377490694583No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5370.11573849565818994No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG5320.11466085603381203No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT5260.1133676884845585No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT5220.11250557678505615No Hit
TCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGA5220.11250557678505615No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT5200.11207452093530498No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTCCCGATGAGCTTT5170.11142793716067821No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5170.11142793716067821No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGT5160.11121240923580263No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT5150.11099688131092705No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC5100.10991924168654912No Hit
GGGATCTAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGG5070.10927265791192235No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTACAGTACCTATC5040.1086260741372956No Hit
CTCTAAGGCTTCTCGATACAGAGCTGACGCGGTGTCTAGGAGATCTCTGA5040.1086260741372956No Hit
TGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCATA4970.1071173786631665No Hit
GTACTGTAGAGGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA4970.1071173786631665No Hit
ATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAAC4960.10690185073829091No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4960.10690185073829091No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA4940.10647079488853974No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT4910.10582421111391298No Hit
GGGTTATTCCCTAAACTTCATGGGTTACATAATTGGAAGTTGGGGAACTT4910.10582421111391298No Hit
CCTAGACACCGCGTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGAGC4910.10582421111391298No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG4870.10496209941441062No Hit
GTTTAGCTTGTATACATGCATACAAAGGCATTAATGCAGGATATCCACAT4870.10496209941441062No Hit
CTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGT4860.10474657148953503No Hit
TCTCGATACAGAGCTGACGCGGTGTCTAGGAGATCTCTGACGGAAGGAAA4840.10431551563978388No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTCCCGATGAGCTTTGC4840.10431551563978388No Hit
GAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAA4840.10431551563978388No Hit
GAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTACAGTGA4820.1038844597900327No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4800.10345340394028152No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4790.10323787601540593No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4760.10259129224077917No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4760.10259129224077917No Hit
AGATATATGAAACCACAATAGTTGCCTGATCTTTAAACCCATGTTAGTAT4750.10237576431590359No Hit
GTGGATATCCTGCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAG4750.10237576431590359No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT4750.10237576431590359No Hit
CTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA4730.10194470846615243No Hit
ATGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCAT4720.10172918054127685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)