FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463977
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG26550.5722266405446822No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT23310.5023955928849921No Hit
GTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT16540.3564831877442201No Hit
TATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT16350.35238815717158395No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT15530.33471486733178585No Hit
GTATACATGCATACAAAGGCATTAATGCAGGATATCCACATTGTGTAAAT15480.3336372277074079No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT15220.32803350166064266No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA14990.32307635938850415No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14770.31833474504124126No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA14740.31768816126661453No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG14630.31531735409298306No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC14020.3021701506755723No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC13900.2995838155770652No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG13890.29936828765218965No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA13310.2868676680094056No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC13280.2862210842347789No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG13050.2812639419626404No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT12900.2780310230895066No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12870.2773844393148798No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT12750.2747981042163728No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGAGT12640.2724272970427414No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC12310.265314875521847No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG12310.265314875521847No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG12130.2614353728740864No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC11180.24096022001090572No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA11130.2398825803865278No Hit
ATTCTATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC10800.23277015886563343No Hit
GTCTAAGGGCAAATATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAA10520.226735376969117No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA9650.207984447504941No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG9610.20712233580543862No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA9530.20539811240643394No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9450.20367388900742928No Hit
TATCCACATTGTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAATTCT9450.20367388900742928No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG9350.2015186097586734No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA8830.1903111576651429No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8720.18794035049151142No Hit
CTCCTAGACACCGCGTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA8220.1771639542477321No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG8210.1769484263228565No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC8200.17673289839798095No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG8180.17630184254822978No Hit
GAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA8110.17479314707410065No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC8100.17457761914922507No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA8070.1739310353745983No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTC7930.1709136444263401No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT7820.16854283725270866No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTT7630.1644478066800725No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7600.16380122290544574No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA7570.16315463913081898No Hit
GGATATCCACATTGTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAAT7450.16056830403231195No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG7370.15884408063330727No Hit
CCATTTACACAATGTGGATATCCTGCATTAATGCCTTTGTATGCATGTAT7220.15561116176017345No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7080.15259377081191525No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT7000.15086954741291056No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT6980.1504384915631594No Hit
ATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT6940.14957637986365702No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC6890.14849874023927911No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT6780.14612793306564767No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT6770.14591240514077206No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC6640.14311054211738944No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA6590.1420329024930115No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCA6490.13987762324425562No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC6450.1390155115447533No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6310.1359981205964951No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGA6250.13470495304724156No Hit
GTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT6230.1342738971974904No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCTTATAGACCACCAAATGCC6190.13341178549798804No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA6120.13190309002385894No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT6120.13190309002385894No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6030.12996333869997867No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC6010.12953228285022747No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6000.12931675492535188No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG5990.12910122700047633No Hit
GCTTTATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTT5930.12780805945122278No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5910.1273770036014716No Hit
ATTTACAAGAGGACATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTC5900.12716147567659603No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT5650.12177327755470636No Hit
CATGCATACAAAGGCATTAATGCAGGATATCCACATTGTGTAAATGGAGC5620.12112669378007962No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT5590.12048011000545286No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTACAG5590.12048011000545286No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG5450.11746271905719463No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT5370.11573849565818994No Hit
CTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5360.11552296773331436No Hit
ATGTGGATATCCTGCATTAATGCCTTTGTATGCATGTATACAAGCTAAAC5330.1148763839586876No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5320.11466085603381203No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTC5280.11379874433430968No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5280.11379874433430968No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT5250.11315216055968291No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT5230.11272110470993174No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAC5160.11121240923580263No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5150.11099688131092705No Hit
GTTTAGCTTGTATACATGCATACAAAGGCATTAATGCAGGATATCCACAT5110.11013476961142471No Hit
CTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGT5100.10991924168654912No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG5070.10927265791192235No Hit
TGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCATA5070.10927265791192235No Hit
ATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGACTCTA5070.10927265791192235No Hit
CTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATTCTAT5060.10905712998704677No Hit
TCCCTAAACTTCATGGGTTACATAATTGGAAGTTGGGGAACTTTGCCACA5050.10884160206217118No Hit
GTACTGTAGAGGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA5040.1086260741372956No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT5030.10841054621242002No Hit
CTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACT5020.10819501828754444No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5020.10819501828754444No Hit
GTGGATATCCTGCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAG5010.10797949036266885No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT5010.10797949036266885No Hit
ATGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCAT4980.10733290658804208No Hit
CATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAA4940.10647079488853974No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTACAGTACCTATC4920.10603973903878856No Hit
CTGCTAGGCTGTACTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTTTA4910.10582421111391298No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4910.10582421111391298No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT4870.10496209941441062No Hit
GAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAA4830.10409998771490829No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG4810.1036689318651571No Hit
ATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAAC4790.10323787601540593No Hit
GTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGAGTAGGATAGAAT4780.10302234809053035No Hit
CTCTAAGGCTTCTCGATACAGAGCTGACGCGGTGTCTAGGAGATCTCTGA4760.10259129224077917No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4740.102160236391028No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA4670.1006515409168989No Hit
GGGTTATTCCCTAAACTTCATGGGTTACATAATTGGAAGTTGGGGAACTT4660.10043601299202332No Hit
GCTAGATTCTATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAAT4650.10022048506714773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)