FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423034
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17620.416514984611166No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA17350.4101325188991901No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGCAAATAATGTCTGGTTT14060.33236099226066934No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG12870.3042308656041831No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGCAAATAATGTCTGGT12230.2891020579906107No Hit
TCCAAAGAGTATGCAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC10970.25931721800138996No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT10480.24773422467224857No Hit
ATATAAGAGGGAATCCACACGTAGCGCATCATTTTGCGGGTCACCATATT9830.23236902943971405No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9510.22480462563292786No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9480.22409546277604164No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA9160.2165310589692554No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC8850.2092030427814313No Hit
GTTTTGATGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG8610.20352973992634163No Hit
TTTCTAGGGGGATCACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAAC8300.1962017237385175No Hit
CTATATAAGAGGGAATCCACACGTAGCGCATCATTTTGCGGGTCACCATA8290.19596533611955538No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8110.19171035897823815No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA8090.19123758374031402No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC8000.1891100951696554No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC7890.18650983136107263No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC7840.18532789326626228No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG7780.18390956755248986No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT7690.18178207898183124No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA7630.18036375326805884No Hit
CCCTCGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGCGGTATTG7540.17823626469740023No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7520.17776348945947607No Hit
ACAGCAAACCTCGCAAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT7520.17776348945947607No Hit
ATATGATCCTGTGGTAATGTTCCCCAACTTCCAATCATGTAACCCATGAA7340.17350851231815884No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT7180.1697263104147657No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA6940.16405300755967606No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6880.16263468184590363No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6860.1621619066079795No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6840.16168913137005536No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6800.1607435808942071No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6760.15979803041835883No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT6760.15979803041835883No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG6640.15696137899081397No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT6630.1567249913718519No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6520.15412472756326914No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6480.15317917708742088No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT6470.1529427894684588No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC6340.1498697504219519No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6040.14277812185308983No Hit
GCACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG5890.1392323075686588No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5870.13875953233073465No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC5830.13781398185488636No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA5700.13474094280837948No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG5560.13143151614291051No Hit
GTTTGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGATGC5560.13143151614291051No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5460.1290676399532898No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5430.1283584770964036No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC5410.12788570185847944No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5280.12481266281197255No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5270.12457627519301048No Hit
GCTCATCGGTACTGACAATTCTGTCGTCCTTTCGCGGAAATATACATCGT5250.12410349995508635No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG5230.12363072471716222No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAATCCACACGTAGCGCA5170.1222123990033898No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5140.12150323614650359No Hit
CTCTCACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA5090.12032129805169324No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA5010.11843019709999669No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG4990.11795742186207256No Hit
GTACAATATGATCCTGTGGTAATGTTCCCCAACTTCCAATCATGTAACCC4970.11748464662414843No Hit
ACAGCATGGGAGGTTGGTCATCAAAACCTCGCAAAGGCATGGGGACGAAT4950.11701187138622426No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAAAGGACGACAGAAT4910.11606632091037601No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG4900.11582993329141392No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4890.11559354567245185No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA4860.11488438281556565No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4810.1137024447207553No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG4800.11346605710179324No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGCAAATAATGTCTGGTTTGGA4790.11322966948283117No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG4760.11252050662594495No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG4750.11228411900698289No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC4740.11204773138802082No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4720.11157495615009669No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4580.1082655294846277No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT4560.10779275424670356No Hit
GTCCCGTGCTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC4560.10779275424670356No Hit
GTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4550.10755636662774151No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4550.10755636662774151No Hit
ATGTATATTTCCGCGAAAGGACGACAGAATTGTCAGTACCGATGAGCTTT4530.10708359138981738No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4520.10684720377085531No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGTAATGTT4460.1054288780570829No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT4410.10424693996227254No Hit
GTGGTCTCCATGCAACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC4330.10235583901057599No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTCAC4300.10164667615368977No Hit
TATTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAG4300.10164667615368977No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC4290.10141028853472771No Hit
GTACTGACAATTCTGTCGTCCTTTCGCGGAAATATACATCGTTTCCATGG4290.10141028853472771No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG4260.10070112567784148No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAGC4250.10046473805887943No Hit
TGTCAGTACCGATGAGCTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCG4240.10022835043991736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)