FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483566
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22930.4741855300000414No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTCT21190.43820285131709014No Hit
ATTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAGAG19150.39601626251638866No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTGCTGTT13830.28600025642828486No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATGAA13590.2810371283340847No Hit
CCTATTAATAATGTCCTCATGTAAATGGATTTTAGGAAAGGAAGGAGTTT12700.2626321949847591No Hit
CTCCAAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG12240.25311953280420874No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGCTCACCCGTG12060.2493971867335586No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11090.2293378773528329No Hit
GTATGTAAATAATGTCGGGTCTGGAAGTAATGATTAACTACTTGATCAGG10780.2229271702311577No Hit
TTATTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAG10680.2208592001919076No Hit
CTCTTCTACTGTCCCTGTCTTTAATCCTGAATGGCAAACTCCTTCCTTTC10350.21403489906238238No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9990.20659020692108213No Hit
TTATTATCCTGATCAAGTAGTTAATCATTACTTCCAGACCCGACATTATT9980.20638340991715712No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT9710.20079989081118194No Hit
GTATAATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCC9700.20059309380725693No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTCCG9660.19976590579155687No Hit
GTACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG9180.18983964960315655No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA9150.18921925859138153No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9050.18715128855213145No Hit
CCATTTACACAATGTGGTTACCCTGCCTTAATGCCTTTGTATGCATGTAT8680.1794997994069062No Hit
GTTCATATACTGTTGATTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8670.17929300240298118No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGCTATTCCCTAAACTTCAT8490.17557065633233107No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTCTGGAAGTAATGATTAACTACT8230.17019393423028087No Hit
GTCTAGAGGCAAATATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAA8180.16915994921065583No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTGTGCCAAG8110.1677123701831808No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT7770.16068127204973054No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7730.15985408403403054No Hit
ATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAATTAATTTCAATCTT7640.15799291099870544No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGATGGC7600.15716572298300543No Hit
GCACTAAATATTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTGAT7240.14972103084170515No Hit
GGATCATATTATACAAAAAATCAAACACTGTTTTAGGAAACTTCCTGTCA7230.14951423383778015No Hit
GTCCCGTACTGGTTGTTGTGGATCCTGGAATTAGAGGACAAACGGGCAAC6750.1395879776493798No Hit
GGTTCATATACTGTTGATTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA6730.13917438364152981No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC6670.13793360161797977No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC6650.13752000761012975No Hit
CATTATTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAA6130.12676656340602938No Hit
ACACCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGG6020.12449179636285429No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGGTAACCACATTGTGTAAAT5810.12014905928042915No Hit
TCCTAAAACAGTGTTTGATTTTTTGTATAATATGATCCTGTGGCAATGTA5800.11994226227650415No Hit
GTAGAAGAGTAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT5750.1189082772568791No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTACTCTTCTACTG5710.11808108924117908No Hit
GACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCC5690.11766749523332905No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG5660.11704710422155402No Hit
CCTCTAGACAAAGGGATTAAACCTTATTATCCTGATCAAGTAGTTAATCA5650.11684030721762903No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT5590.11559952519407898No Hit
TCCCGATACAGAGCTGAGGCTGTATCAAGTAGATCCCGGACTGAAGGAAA5550.11477233717837895No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTACTCTTCTACTGTCCCTGTC5520.11415194616660393No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA5500.11373835215875393No Hit
CTAGCTACCTGGGTGGGTAATAATTTGGAAGATCCTGCATCCAGGGATCT5460.1129111641430539No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA5320.1100160060881038No Hit
GGGTAAAGGTTCATATACTGTTGATTTAGAAAGGCCTTGTAAGTTGGCGA5250.10856842706062876No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTGTGCCAAGTG5230.10815483305277873No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5210.1077412390449287No Hit
GTTTAGGGAATAGCCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC5160.10670725402530368No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAG5150.10650045702137868No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCTGTATCAAGTAGATCCCGGA5140.10629366001745366No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5130.10608686301352865No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT5120.10588006600960366No Hit
ACTTGATACAGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC5120.10588006600960366No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5080.10505287799390363No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCAGGATCTTCCAAATT5050.1044324869821286No Hit
ATGTAAGACCTTGGGCAGGATCGGATGGGCGTTCACGGTGGTCTCCATGC5010.10360529896642859No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA4970.10277811095072853No Hit
CCACAGTAGTTGTCTGATTTTTAGGCCCATGTTAGTATTAACATAATTGA4950.10236451694287854No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAAATCCATTTACATG4870.10071014091147848No Hit
GCTTTGGGGCATGGACATTGACCTTTATAAAGAATTTGGAGCTACTGTGG4860.10050334390755346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)