FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370790
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13310.3589632945872327No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12590.3395452951805604No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12040.32471210118935245No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT10460.282100380269155No Hit
ATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTCTGCCAAG10220.2756277138002643No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10070.2715822972572076No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA8940.2411068259661803No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8550.23058874295423287No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT8350.22519485423015723No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8140.2195312710698778No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8020.21629493783543244No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7820.21090104911135685No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7710.20793441031311524No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7670.2068556325683001No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7670.2068556325683001No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7260.19579816068394507No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC7190.19391029963051862No Hit
CCCTTAACTTCATGGGTTATGTAATTGGAAGTTGGGGTACTTTACCGCAG7020.18932549421505435No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6650.17934680007551443No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6490.17503168909625394No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6490.17503168909625394No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6480.17476199466005016No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6310.17017718924458589No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6180.16667116157393672No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT5790.15615307856198926No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT5770.1556136896895817No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5730.1545349119447666No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5640.15210766201893255No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT5360.14455621780522668No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5240.14131988457078132No Hit
CTCCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5180.13970171795355862No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC4980.134307829229483No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC4980.134307829229483No Hit
GATGATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC4970.13403813479327922No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTTCACCAAGCTCTGCTAG4800.12945332937781495No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4780.1289139405054074No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4750.12810485719679604No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4740.12783516276059226No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4660.125677607270962No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTATTCG4630.12486852396235067No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4540.12244127403651664No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4540.12244127403651664No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC4480.12082310741929395No Hit
GCAGTAGGAGTGCGAATCCACACTCCGAAAGATACCAAATACTCAAGAAC4460.12028371854688638No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA4410.11893524636586747No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA4410.11893524636586747No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA4410.11893524636586747No Hit
GGATTAAAGACAGGTACGGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC4360.11758677418484856No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4310.11623830200382966No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4290.1156989131314221No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4280.11542921869521833No Hit
CTACCGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4260.11488982982281075No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4220.11381105207799563No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG4210.11354135764179184No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4150.11192319102456916No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGGTGGCC4130.1113838021521616No Hit
CTCCGAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4120.11111410771595781No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA4100.11057471884355025No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4100.11057471884355025No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC4060.10949594109873513No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4040.10895655222632757No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA4030.10868685779012378No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4020.10841716335392002No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG4010.10814746891771622No Hit
CCCTTAGACAAAGGCATTAAACCGTATTATCCTGAACATGCAGTTAATCA3960.10679899673669734No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA3950.10652930230049354No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC3910.10545052455567842No Hit
GGATGGGGCTTGGCCATAGGCCACCAGCGCATGCGTGGAACCTTTGTGGC3890.10491113568327085No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA3870.10437174681086331No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATAACCCATGAA3860.10410205237465951No Hit
GTACAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT3860.10410205237465951No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT3860.10410205237465951No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3850.10383235793845573No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3820.1030232746298444No Hit
GATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGC3790.10221419132123305No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA3790.10221419132123305No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3790.10221419132123305No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC3780.10194449688502925No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG3780.10194449688502925No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTCTGCCAAGTGTTTGCTG3770.10167480244882549No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT3740.10086571914021414No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3740.10086571914021414No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT3730.10059602470401036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)