FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472016
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50491.069667129927799No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG12440.26355038812243653No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10120.2143995118809532No Hit
ATCCTAACCTTACTAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT10050.21291651130470154No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9660.20465407952272807No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9500.20126436391986713No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8300.17584149689841022No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7960.16863835124233076No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7900.16736720789125795No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCACAG7630.16164706281143013No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7430.15740991830785397No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6850.14512219924748315No Hit
ATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCCATGAA6800.1440629131215891No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGA6760.14321548422087388No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6670.1413087691942646No Hit
CATGTATACAATCTAAGCAAGCTTTTACTTTCTCGCCAACTTACAAGGCT6610.14003762584319177No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC6440.13643605301515205No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6370.13495305243890038No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTGGACAAAGGCATTAAAC6070.12859733568353615No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA6060.12838547845835735No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT5900.12499576285549642No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTTGGGGAGGAGATTA5850.12393647672960238No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5850.12393647672960238No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5730.1213941900274567No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5540.11736890274905934No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5470.1158859021728077No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCGCG5350.11334361547066202No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5350.11334361547066202No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5170.10953018541744348No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCAGTGTCGAGGAGATCTCGAA5150.10910647096708587No Hit
GTACAATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCC5130.10868275651672824No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5110.10825904206637063No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTAGTAAGGTT5090.10783532761601301No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5040.10677604149011899No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTAGTA5040.10677604149011899No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATA5010.10614046981458256No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5000.10592861258940375No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4910.10402189756279448No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4820.1021151825361852No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4760.10084403918511235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)