FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472016
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50411.0679722721263687No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG12740.26990610487780076No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9600.2033829361716552No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9220.1953323616148605No Hit
ATCCTAACCTTACTAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT9220.1953323616148605No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8920.18897664485949628No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8450.17901935527609233No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8100.17160435239483407No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7700.16313006338768177No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCACAG7320.1550794888308871No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7150.15147791600284738No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6850.14512219924748315No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC6770.14342734144605268No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGA6640.1406731975187282No Hit
ATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCCATGAA6400.1355886241144368No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6260.13262262296193347No Hit
CATGTATACAATCTAAGCAAGCTTTTACTTTCTCGCCAACTTACAAGGCT6160.13050405071014543No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCGCG6150.1302921934849666No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTTGGGGAGGAGATTA6060.12838547845835735No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5930.12563133453103284No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTGGACAAAGGCATTAAAC5860.1241483339547812No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT5850.12393647672960238No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA5790.12266533337852954No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5790.12266533337852954No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5640.11948747500084743No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTAGTAAGGTT5530.11715704552388055No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5480.11609775939798651No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5280.11186061489441036No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5270.11164875766923155No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATA5230.11080132876851631No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTAGTA5230.11080132876851631No Hit
GTACAATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCC5220.11058947154333751No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5150.10910647096708587No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5110.10825904206637063No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCAGTGTCGAGGAGATCTCGAA5080.10762347039083421No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5070.10741161316565541No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5000.10592861258940375No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4980.10550489813904614No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4940.10465746923833091No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4870.10317446866207926No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4760.10084403918511235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)