FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549134
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44490.8101847636460318No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA36100.6573987405624128No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT24930.45398755130805957No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG21490.391343460794633No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA21050.38333084456617145No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT20670.3764108578234092No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC20580.3747719135948603No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT19240.35036985508090923No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA17830.32469306216697563No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC17400.3168625508527973No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC15450.28135209256757004No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT15450.28135209256757004No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA13610.24784478833945814No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT13610.24784478833945814No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA13590.24748057851089167No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG13320.24256374582524487No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTGCATCCTGCTGCTATGCCT12670.23072692639683573No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC12570.2289058772540036No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT12340.22471746422548958No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGGACCTCTATG12170.2216216806826749No Hit
CAGACACATCCAGCGATAACCAGGACAAATTGGAGGACAACAGGTTGGTG11380.20723539245430075No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT11150.20304697942578678No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC11030.20086172045438816No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA10960.19958698605440564No Hit
AGTTCAGGAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTC10710.19503436319732523No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG10680.1944880484544756No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACACGCAAAACAG10430.18993542559739518No Hit
ACATAGAGGTCCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG10320.18793227154027978No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT10100.18392596342604903No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10050.18301543885463292No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA10040.18283333394034973No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG9810.17864492091183573No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9800.1784628159975525No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG9750.1775522914261364No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT9600.17482071771188817No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC9510.1731817734833392No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC9440.1719070390833567No Hit
GTTTTGCGTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT9410.17136072434050703No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG9350.17026809485480776No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT9120.16607968182629376No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC9110.16589757691201054No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT9030.1644407375977448No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA8930.16261968845491265No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC8870.16152705896921335No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG8870.16152705896921335No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC8860.16134495405493013No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT8820.16061653439779724No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8810.16043442948351405No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG8700.15843127542639865No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG8580.15624601645500005No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA8470.15424286239788468No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA8470.15424286239788468No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8390.15278602308361894No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC8390.15278602308361894No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA8320.15151128868363642No Hit
TTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGA8320.15151128868363642No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA8220.14969023954080424No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC8140.14823340022653853No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT8140.14823340022653853No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA8050.14659445599798956No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG7930.14440919702659094No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA7890.14368077736945808No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT7860.14313446262660842No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACACGCAAAAC7770.1414955183980595No Hit
GCTTTAATGCCTTTATATGCATGTATACACGCAAAACAGGCTTTTACTTT7640.13912815451237767No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC7370.13421132182673082No Hit
TACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG7350.1338471119981644No Hit
ATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT7300.1329365874267483No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG7220.13147974811248256No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT7200.13111553828391612No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG7090.12911238422680074No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG7010.127655544912535No Hit
CTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTG6980.12710923016968537No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC6980.12710923016968537No Hit
GTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT6870.12510607611257No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA6860.12492397119828674No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC6780.12346713188402102No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6780.12346713188402102No Hit
GTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAA6770.12328502696973781No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6760.12310292205545458No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6740.12273871222688816No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA6640.12091766308405599No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG6570.11964292868407347No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG6450.11745766971267485No Hit
CACTTGGCACAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT6430.11709345988410844No Hit
CATGTATACACGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6210.11308715176987766No Hit
CTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTG6190.11272294194131123No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGACTCAAGATG6140.11181241736989514No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6080.11071978788419586No Hit
GGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA6020.10962715839849654No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTTT5950.10835242399851402No Hit
GGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAA5850.10653137485568186No Hit
CATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAA5850.10653137485568186No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT5800.10562085028426578No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC5790.10543874536998254No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5730.10434611588428326No Hit
ATCTTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT5620.10234296182716786No Hit
CCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAA5570.10143243725575178No Hit
ATGTTGTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG5570.10143243725575178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)