FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549134
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44150.8039931965604024No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA37840.6890849956476925No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG24670.44925282353669593No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA22920.41738446353713304No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT21860.39808134262311207No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC20690.37677506765197566No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT19790.36038562536648616No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT18370.33452672753826934No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC17830.32469306216697563No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA16380.2982878495959092No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC15730.2864510301675001No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT14720.2680584338248952No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA14250.25949950285358403No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA13770.2507584669679896No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTGCATCCTGCTGCTATGCCT13340.24292795565381128No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT12920.23527954925391617No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGGACCTCTATG11710.2132448546256469No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC11530.20996696616854904No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACACGCAAAACAG11070.20159014011152104No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT11050.20122593028295463No Hit
GTTTTGCGTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT10930.19904067131155603No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC10890.19831225165442312No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT10820.19703751725444063No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT10800.1966733074258742No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG10640.1937596287973427No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC10580.1926669993116434No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG10510.1913922649116609No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA10480.19084595016881126No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG10420.18975332068311196No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT10360.18866069119741266No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA10250.18665753714029726No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC10220.18611122239744762No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA10200.18574701256888118No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT10060.18319754376891614No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG9880.17991965531181825No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG9830.17900913074040217No Hit
AGTTCAGGAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTC9820.17882702582611892No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC9610.1750028226261714No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG9550.1739101931404721No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC9470.17245335382620636No Hit
ACATAGAGGTCCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG9300.16935757028339166No Hit
CAGACACATCCAGCGATAACCAGGACAAATTGGAGGACAACAGGTTGGTG9190.16735441622627628No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC9080.1653512621691609No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8680.1580670655978322No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG8670.157884960683549No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA8570.15606391154071683No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA8530.15533549188358398No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8500.1547891771407343No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA8410.15315023291218535No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT8390.15278602308361894No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC8320.15151128868363642No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT8300.15114707885506998No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG8200.1493260297122378No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG8150.14841550514082175No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACACGCAAAAC8110.14768708548368886No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA8080.14714077074083923No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT8050.14659445599798956No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA7960.1449555117694406No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA7910.14404498719802453No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA7810.14222393805519234No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC7800.14204183314090915No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT7770.1414955183980595No Hit
GCTTTAATGCCTTTATATGCATGTATACACGCAAAACAGGCTTTTACTTT7750.14113130856949305No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC7680.13985657416951053No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG7610.138581839769528No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC7540.1373071053695455No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC7540.1373071053695455No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT7440.13548605622671334No Hit
CTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTG7350.1338471119981644No Hit
ATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT7280.13257237759818188No Hit
GTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT7270.13239027268389864No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG7050.12838396456966789No Hit
TTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGA7010.127655544912535No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA6890.1254702859411364No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6790.12364923679830424No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC6780.12346713188402102No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6620.12055345325548955No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6610.12037134834120633No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6600.12018924342692312No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA6560.11946082376979025No Hit
CTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTG6530.1189145090269406No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG6490.11818608936980775No Hit
CACTTGGCACAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT6490.11818608936980775No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG6330.11527241074127627No Hit
GTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAA6200.11290504685559444No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC6100.11108399771276227No Hit
CATGTATACACGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5910.10762400434138117No Hit
ATGTTGTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG5890.10725979451281471No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG5870.1068955846842483No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5840.10634926994139865No Hit
TACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG5810.10580295519854899No Hit
CATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAA5630.10252506674145109No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5620.10234296182716786No Hit
GGATATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACA5620.10234296182716786No Hit
GTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA5570.10143243725575178No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTTT5560.10125033234146857No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGACTCAAGATG5510.10033980777005248No Hit
CCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAA5500.10015770285576928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)