FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002490205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74530
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT7000.9392191064001073No Hit
GAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTTCCTGTGGC6350.8520059036629545No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5500.7379578693143701No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4440.5957332617737824No Hit
GTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTTCCTGTGGCAAG4380.5876828122903529No Hit
GTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCA4150.5568227559372064No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTATACCGCTAT4100.5501140480343486No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT4090.548772306453777No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGGGTAAAG4070.5460888232926339No Hit
GGGTAAAGGGAGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT3670.49241916006977055No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTCTCTAGGGGGAGCACCCGCG3410.45753387897490944No Hit
GCAATGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAG3340.44814168791090836No Hit
ACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAAC3190.42801556420233466No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG3020.40520595733261777No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT2980.39983899101033143No Hit
GTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTTCCTGTGGCAAT2930.39313028310747355No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT2920.3917885415269019No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA2680.359586743593184No Hit
GCCCAGAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT2650.3555615188514692No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCG2560.343485844626325No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2520.33811887830403864No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC2500.3354353951428955No Hit
CATTTAAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT2470.33141017040118076No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTATACC2380.3193344961760365No Hit
AGTCTGTACAACATCTTGAATCCCTTTATACCGCTATTACCAATTTTCTT2380.3193344961760365No Hit
GGCAATGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTA2360.31665101301489335No Hit
GTACCTTGCCACAGGAACATATTGTACAAAAAATCAAACAATGTTTTCGG2260.30323359720917753No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAA2250.30189185562860593No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG2130.285790956661747No Hit
GTACAACATCTTGAATCCCTTTATACCGCTATTACCAATTTTCTTTTGTC2090.28042399033946064No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2040.2737152824366027No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC2010.26969005769488796No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT1940.2602978666308869No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA1910.25627264188917215No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTTAAATGTATA1910.25627264188917215No Hit
GTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTTAAATGTATAC1890.25358915872802895No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC1840.2468804508251711No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG1780.2388300013417416No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG1760.2361465181805984No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG1760.2361465181805984No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1750.23480477660002683No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC1740.23346303501945526No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGT1700.22809606869716895No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC1700.22809606869716895No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT1690.22675432711659732No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG1690.22675432711659732No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA1670.22407084395545418No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA1670.22407084395545418No Hit
GCTTGTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGGGT1570.21065342814973836No Hit
CCCTCATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATG1560.2093116865691668No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTATAAA1540.2066282034080236No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT1530.20528646182745203No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC1520.20394472024688046No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT1520.20394472024688046No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG1510.20260297866630889No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGC1480.19857775392459412No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG1460.19589427076345095No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA1430.1918690460217362No Hit
CTCCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCGCCGAATATG1420.19052730444116464No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTC1400.18784382128002147No Hit
CATGCATACAAAGGCATTAATGCTGGGTAACCACATTGGGTAAAGGGAGC1390.18650207969944987No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG1390.18650207969944987No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA1390.18650207969944987No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG1390.18650207969944987No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA1340.179793371796592No Hit
CATTAATGCTGGGTAACCACATTGGGTAAAGGGAGCGGCAAAGCCCAGAA1330.1784516302160204No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC1320.1771098886354488No Hit
ATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT1310.17576814705487723No Hit
CCCTTTACCCAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT1270.17040118073259092No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA1240.16637595599087615No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC1230.16503421441030458No Hit
ATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAG1230.16503421441030458No Hit
GTTTTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT1230.16503421441030458No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC1210.1623507312491614No Hit
GTCCCGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA1200.16100898966858984No Hit
GACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACAC1200.16100898966858984No Hit
GGGTAACCACATTGGGTAAAGGGAGCGGCAAAGCCCAGAAGACCCACAAT1200.16100898966858984No Hit
CTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA1200.16100898966858984No Hit
GCCCCAACCAGTGGGAGTTGCGTCAGCAAACACTTGGCAAAGACCTGACC1190.15966724808801824No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA1170.1569837649268751No Hit
CAATTCTCTAGGGGGAGCACCCGCGTGTCCTGGCCAAAATTTGCAGTCCC1170.1569837649268751No Hit
CCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAG1150.1543002817657319No Hit
CCTACAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT1150.1543002817657319No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG1140.15295854018516034No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC1130.15161679860458877No Hit
GTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC1130.15161679860458877No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT1110.1489333154434456No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG1110.1489333154434456No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTCTCTAGGG1100.14759157386287403No Hit
ATATGTTCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATGAA1090.14624983228230243No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG1090.14624983228230243No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT1090.14624983228230243No Hit
GCATTAATGCTGGGTAACCACATTGGGTAAAGGGAGCGGCAAAGCCCAGA1090.14624983228230243No Hit
GGCCAAGTCTGTACAACATCTTGAATCCCTTTATACCGCTATTACCAATT1030.13819938279887295No Hit
CCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATG1020.13685764121830135No Hit
CCCGCGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC1020.13685764121830135No Hit
GTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATA1010.13551589963772978No Hit
ACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCGAGGACT1010.13551589963772978No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTATACCGC1000.13417415805715818No Hit
GTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA990.1328324164765866No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG990.1328324164765866No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGAGTTGCGTCAGCAAACACTTGGC990.1328324164765866No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGCTTGGTTTTA970.13014893331544344No Hit
ACCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCAC960.12880719173487187No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTATAA960.12880719173487187No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC960.12880719173487187No Hit
GGTAATAGCGGTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCA950.12746545015430027No Hit
CCCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTA940.1261237085737287No Hit
GCTTAGCTTGTATACATGCATACAAAGGCATTAATGCTGGGTAACCACAT940.1261237085737287No Hit
GTGAAAGCCTGCTTAGCTTGTATACATGCATACAAAGGCATTAATGCTGG940.1261237085737287No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT940.1261237085737287No Hit
CACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAA930.12478196699315712No Hit
CTTCCAGGAACATCAACTACCAGCACGGGACCATGCAAGACCTGCACGAT930.12478196699315712No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA930.12478196699315712No Hit
CCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAAT920.12344022541258555No Hit
CTGTACAACATCTTGAATCCCTTTATACCGCTATTACCAATTTTCTTTTG920.12344022541258555No Hit
ACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGAC920.12344022541258555No Hit
GTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCGAG900.12075674225144237No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA890.1194150006708708No Hit
GCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAG890.1194150006708708No Hit
GGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGAG880.1180732590902992No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG880.1180732590902992No Hit
GTACAATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCC880.1180732590902992No Hit
CTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTG870.11673151750972763No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT870.11673151750972763No Hit
GACTACTGCCTCTCCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTG870.11673151750972763No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAGCGTTGGGGCTACT860.11538977592915604No Hit
GTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGC850.11404803434858447No Hit
CTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA850.11404803434858447No Hit
GATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCCTATGG850.11404803434858447No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA840.11270629276801288No Hit
GATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC840.11270629276801288No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC840.11270629276801288No Hit
TGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCAT830.1113645511874413No Hit
CCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA830.1113645511874413No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTCTCTAGGGGG830.1113645511874413No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT820.11002280960686972No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA810.10868106802629812No Hit
CTGATGGCCTATGGCCAAGCCCCAACCAGTGGGAGTTGCGTCAGCAAACA800.10733932644572655No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG800.10733932644572655No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG800.10733932644572655No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAG790.10599758486515498No Hit
GGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTT790.10599758486515498No Hit
CAATTCTCTAGGGGGAGCACCCACGTGTCCTGGCCAAAATTTGCAGTCCC770.1033141017040118No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTGCCACAG760.10197236012344023No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGCGGTATAAAGGGATTCAAGATG760.10197236012344023No Hit
CCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT760.10197236012344023No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG760.10197236012344023No Hit
CTTGTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGGGTA760.10197236012344023No Hit
TGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCTCAT760.10197236012344023No Hit
GTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCGAGGACTGGGGA750.10063061854286864No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT750.10063061854286864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)