FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297691
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20080.6745249268536838No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA10400.3493555398046968No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9260.31106079794148966No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8990.30199099065809853No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8350.2804921882085787No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8280.27814075669066246No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA7820.2626884924300701No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7640.25664195424114267No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7630.2563060354528689No Hit
ATATGTTCCTGTGGCAATGTACCCCAACTCCCAATTACATATCCCATGAA7400.2485799033225727No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7190.24152560876882406No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6730.2260733445082317No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6320.21230067418900808No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6280.21095699903591306No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6250.20994924267109186No Hit
ACTCACAACTGTGCCAACAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6200.2082696487297231No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA5360.18005247051472836No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5260.17669328263199088No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5050.1696389880782422No Hit
GGAACATATTGTACAAAAAGTCAAAATGTGTTTTAGAAAACTTCCTGTAA5040.16930306928996847No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC4890.16426428746586225No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGACTTTTTGTACAATATGTT4870.16359244988931476No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG4860.163256531101041No Hit
GCACCGGACCGTGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4860.163256531101041No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4790.1609050995831248No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA4700.15788183048866106No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4700.15788183048866106No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGACTTTTTGT4570.15351488624110235No Hit
ATATGCAGGTGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4560.1531789674528286No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4530.15217121108800738No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4420.14847610441699616No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT4360.14646059168735368No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA4330.14545283532253242No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG4250.14276548501634245No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4220.1417577286515212No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4030.13537527167432No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG3970.13335975894467755No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG3910.13134424621503504No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3890.13067240863848756No Hit
GGGTAAAGGTTCAGATATTGTTTACGGAGAAAGGCCTTGTAAGTTGGCGA3860.12966465227366633No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT3700.12428995166128635No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3690.12395403287301261No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA3630.12193852014337014No Hit
ATGTAATTGGGAGTTGGGGTACATTGCCACAGGAACATATTGTACAAAAA3610.12126668256682266No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC3590.12059484499027515No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA3550.11925116983718016No Hit
GTCTATATGCAGGTGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT3540.11891525104890642No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG3500.11757157589581144No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3470.11656381953099018No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA3440.11555606316616894No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTCAATGGCAAACTCCTT3440.11555606316616894No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA3410.11454830680134771No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGTACATTGCCACAG3380.11354055043652647No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGACTTGTGGGTCT3370.1132046316482527No Hit
CCACCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3300.11085320013033649No Hit
GGATATCCACATTGCGTAAAAGGGGCTGCAAACCCCAAAAGACCCACAAG3300.11085320013033649No Hit
GTACAATATGTTCCTGTGGCAATGTACCCCAACTCCCAATTACATATCCC3270.10984544376551526No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAATCCACACGTAGCGCC3210.10782993103587277No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC3200.10749401224759902No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG3170.10648625588277778No Hit
GCGTAAAAGGGGCTGCAAACCCCAAAAGACCCACAAGTCGTTGACATACT3160.10615033709450404No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT3160.10615033709450404No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG3150.1058144183062303No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAAC3150.1058144183062303No Hit
TATCCACATTGCGTAAAAGGGGCTGCAAACCCCAAAAGACCCACAAGTCG3150.1058144183062303No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG3150.1058144183062303No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3130.10514258072968279No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGGTGA3110.10447074315313529No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3090.1037989055765878No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3070.10312706800004032No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC3070.10312706800004032No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3050.10245523042349282No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG3050.10245523042349282No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3030.1017833928469453No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTTCTAAAAC3030.1017833928469453No Hit
CGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT3020.10144747405867156No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3010.10111155527039782No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3000.10077563648212408No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG3000.10077563648212408No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT2980.10010379890557658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)