FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323329
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13580.4200056289414188No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG11250.347942807480925No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG10120.31299388548506324No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGGGTAAAG9280.2870141558598208No Hit
CATGTATACAGGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8500.26289012120781No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7990.2471167139353414No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7920.2449517364665712No Hit
GTACCGGAGCTCCACCGCACGGCCTGAGGATGACTGTCTCTTAGAGGTGG7840.2424774765022624No Hit
CTCCAGCACAGTCCACCAAGCTCTGCCTGCTGGTGGCTCCAGTTCCGGAA7340.2270133517253324No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGT6930.2143327694082498No Hit
GTATAAAGAGTTTGGAGCCTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6530.2019614695867058No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6400.19794079714470397No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG6300.194847972189318No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG6290.19453868969377938No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG6100.188662322278546No Hit
CCTCCCAATTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5880.1818581073766968No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5840.18062097739454241No Hit
CATTTAAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT5740.1775281524391564No Hit
GTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATT5680.1756724574659248No Hit
ATCCTAAACTTACCAAATATTTGCCTTTAGATAAAGGCATTAAACCTTAT5420.1676311125819212No Hit
GTTGTCAATATGCCTTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5390.1667032650953054No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5250.162373310157765No Hit
GGGTAAAGGGAGCTGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT5230.1617547451666878No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5130.1586619202113018No Hit
GTGCTGGAGTTCCACCGCACGGCCTGAGGATGACTGTCTCTTAGAGGTGG5120.1583526377157632No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAATCTACT5060.15649694274253162No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC5020.1552598127603772No Hit
ATATAAGAGAGAATCTACTCACAGCGCCTCATTTTGTGGGTCACCATATT5020.1552598127603772No Hit
GGTTTGCAGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA4970.1537134002826842No Hit
ATATTAACGTTGACATAGCTTACTACTGCTTCCCTGGATGCTGGGTCTGG4940.1527855527960684No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4920.15216698780499122No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAGGCAAATATTTGGTA4910.1518577053094526No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA4890.1512391403183754No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4860.1503112928317596No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTACTCGGCAACGGTCAGGTC4840.14969272784068238No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTACAA4810.1487648803540666No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4680.1447442079120648No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4620.1428885129388332No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4600.142269947947756No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGA4540.1404142529745244No Hit
GCATTAATGCCTTTGTATGCATGTATACAGGCTAAGCAGGCTTTCACTTT4520.13979568798344721No Hit
CCCTTTACCCAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT4460.1379399930102156No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4460.1379399930102156No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAAGGCAAATATTTGGTAAGTTT4420.1367028630280612No Hit
GCTTACTACTGCTTCCCTGGATGCTGGGTCTGGCAAATTACTTCCCACCC4230.1308264956128278No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4220.1305172131172892No Hit
CCTTTACCCCGTTACTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4210.1302079306217506No Hit
TTCTTCTACTGTTCCTGTCTTTAATCCTGAATGGAAAACTCCCTCTTTTC4200.129898648126212No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4110.1271151056663646No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAATCTACTC4070.1258779756842102No Hit
CCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG4060.1255686931886716No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG4010.1240222807109786No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT3920.1212387382511312No Hit
CTGCCAACCAGGTAGGAGTGGGATCATTCGGGCCAGGGTTCACCCCACCA3910.1209294557555926No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3860.11938304327789959No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3850.11907376078236101No Hit
GTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC3850.11907376078236101No Hit
CTCAAGAACAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3780.1169087833135908No Hit
ATCTGAACCTTTACCCCGTTACTCGGCAACGGTCAGGTCTTTGCCAAGTG3780.1169087833135908No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCAATTACAGACCACCAAATGCC3750.115980935826975No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG3740.11567165333143639No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3730.11536237083589779No Hit
GGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA3690.11412524085374341No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT3670.1135066758626662No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3670.1135066758626662No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCT3630.1122695458805118No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCAA3600.11134169839389599No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA3560.11010456841174161No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3510.1085581559340486No Hit
GCACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAAACTCTACG3480.1076303084474328No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3460.10701174345635558No Hit
ATCTTGCCACCTGGGTGGGAAGTAATTTGCCAGACCCAGCATCCAGGGAA3460.10701174345635558No Hit
CCCCAAAAGATTGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGATCCCA3450.106702460960817No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA3400.105156048483124No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA3390.1048467659875854No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC3370.1042282009965082No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC3360.1039189185009696No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3340.10330035350989239No Hit
CTATATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAGGCAAATAT3330.10299107101435379No Hit
CTCCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCGCCGAAAATG3310.1023725060232766No Hit
CCTCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG3260.1008260935455836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)