FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356079
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC13130.368738397939783No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGTAAGTATTTGGTAACATT12710.3569432625906049No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAAAGTCCAA12160.3414972520143002No Hit
CCTACTGCATATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA12010.337284703675308No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT11290.3170644716481455No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10480.2943167106175877No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC10390.29178918161419237No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9490.26651389158023925No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9340.26230134324124704No Hit
ATCTAAGGGTAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA9060.25443791967512824No Hit
ACCAAATACTTACCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA8930.2507870444480017No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA8840.24825951544460637No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8400.23590270698356264No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG8230.23112848553270482No Hit
GGTGTAAGCAATTTGTGGGATCCCTAACAGTAAATGAAAACAGGAGATTA7900.221860879186922No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA7870.2210183695191236No Hit
GTATAATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATCACATATCCC7820.21961418673945948No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA7330.20585319549875167No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATA7320.20557235894281886No Hit
GTTGATAGGTGTAAGCAATTTGTGGGATCCCTAACAGTAAATGAAAACAG7250.2036065030512892No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7240.20332566649535638No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7090.19911311815636418No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATCACATATCCCATGAA7060.19827060848856573No Hit
CCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA7010.1968664257089017No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC6760.18984551181058135No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6210.17439950123427667No Hit
CATGTATACAAGATAAACAGGCTTTTACCTTTTCGCCAACTTACAAGGCC6160.1729953184546126No Hit
CACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG6100.17131029911901574No Hit
GTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCGTTGACATACT6080.1707486260071501No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5990.1682210970037548No Hit
GCTTTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTT5910.16597440455629228No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5860.16457022177662822No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5860.16457022177662822No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5820.16344687555289697No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC5800.16288520244103136No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG5790.16260436588509852No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5710.16035767343763602No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5710.16035767343763602No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5690.1597960003257704No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT5610.1575493078783079No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG5560.15614512509864384No Hit
CAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAACA5490.15417926920711414No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5490.15417926920711414No Hit
TCTATATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATT5470.15361759609524853No Hit
CTCCTACTGCATATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCG5460.15333675953931572No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA5420.15221341331558447No Hit
GTTGTGAGTGTGCCCAGAGCCTGAGGGCTCCACCCCAACAATCCCCCATG5410.15193257675965166No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5400.15165174020371885No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA5300.1488433746443907No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT5260.14772002842065945No Hit
ATATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5240.14715835530879384No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG5160.14491166286133134No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5130.1440691531935329No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTAAC4960.13929493174267507No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4910.137890748963011No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAGGTAA4900.1376099124070782No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4890.13732907585114537No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATCATT4880.1370482392952126No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4830.1356440565155485No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4830.1356440565155485No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4810.1350823834036829No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4720.13255485440028758No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4710.13227401784435477No Hit
CTGTTAGGGATCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGC4710.13227401784435477No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA4660.1308698350646907No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4660.1308698350646907No Hit
ATCCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTA4590.12890397917316102No Hit
ACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATT4410.12384892116637039No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGATAAACAGGCTTTTACCTT4400.12356808461043757No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4380.12300641149857194No Hit
GCTCTGGATAATAGGGTTTGATTCCTTTATCTAAGGGTAAGTATTTGGTA4360.12244473838670632No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC4280.12019804593924382No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4270.119917209383311No Hit
ACTACATGCTCTGGATAATAGGGTTTGATTCCTTTATCTAAGGGTAAGTA4250.11935553627144538No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGATCATTAACCTAATCTCCTCC4240.11907469971551257No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCGGCAATGTCAACGACCGACCT4210.11823219004771413No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG4190.1176705169358485No Hit
GCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTT4130.11598549760025163No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAT4130.11598549760025163No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4120.11570466104431881No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACCCCCATAGGTAT4080.11458131482058756No Hit
GATTTAAATGGATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4070.11430047826465475No Hit
CCTTTATCTAAGGGTAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG4050.11373880515278914No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4040.1134579685968563No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATACAAGATAAAC4040.1134579685968563No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4040.1134579685968563No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA3990.11205378581719225No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3990.11205378581719225No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGGTT3970.11149211270532662No Hit
TAACCACATTGTGTAAAAGGGGCAGCGAATCCCAAAAGACCCACAATTCG3930.11036876648159537No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC3930.11036876648159537No Hit
CATATAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCG3920.11008792992566258No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCT3910.10980709336972974No Hit
GTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCA3900.10952625681379695No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3890.10924542025786413No Hit
GCATATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGAAACTAC3850.10812207403413288No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACACGTAGCGCC3820.10727956436633443No Hit
CTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCT3790.10643705469853601No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTC3780.10615621814260318No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA3700.10390952569514068No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA3670.10306701602734224No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3630.10194366980361098No Hit
CCTACTGCATATAGACCACCAAATATTCAAGAACAGTTTCTCTTCCAAAA3620.10166283324767819No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACTGCATATAGACCACCAAATGCC3580.10053948702394694No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3580.10053948702394694No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC3570.10025865046801412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)