FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576288
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33000.5726303514909212No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA21960.3810594702648676No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT21070.3656158032095064No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA21010.3645746571158865No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATACCCCATGAA17890.310435060247654No Hit
CCCTTAACTTCATGGGGTATGTAATTGGAAGTTGGGGTACTTTACCACAG17880.31026153589871736No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC17630.30592342717530124No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT16510.28648870009439725No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT13600.23599311455383418No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA13530.23477844411127768No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA13280.2304403353878616No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA12790.22193764228996612No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12670.21985535010272642No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC12650.21950830140485314No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC12410.2153437170303737No Hit
GTACACGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA12200.21169970570270422No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA11810.20493225609417515No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA11150.19347964906435672No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG11150.19347964906435672No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC10820.1877533455494475No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10820.1877533455494475No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATACCCC10740.18636515075795435No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG10270.17820950635793215No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT10150.17612721417069244No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA10140.1759536898217558No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA10100.17525959242600922No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9790.1698803376089733No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATACCCCATGAAGTTAAGGGAG9780.16970681326003664No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT9740.16901271586429006No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9650.16745099672386027No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9590.16640985063024044No Hit
GATTTTTAGGCCCATATTAACATTGACATAGCTGACTACTAATTCCCTGG9450.16398050974512743No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG9440.1638069853961908No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT9180.15929535232383807No Hit
GTCTGCACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT9160.1589483036259648No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATATGTT9140.15860125492809152No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGT9110.15808068188128158No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT9010.15634543839191517No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAAT8900.15443667055361207No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC8690.1507926592259426No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8690.1507926592259426No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA8600.1492309400855128No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG8590.14905741573657616No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT8540.14818979399189294No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8520.14784274529401967No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8400.14576045310677996No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG8290.14385168526847686No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA8210.14246349047698373No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTT8130.14107529568549057No Hit
CTTTACCACAGGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAA8130.14107529568549057No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC8060.13986062524293408No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7960.13812538175356764No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG7960.13812538175356764No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT7950.137951857404631No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7920.1374312843578211No Hit
ATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA7870.13656366261313788No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT7850.1362166139152646No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT7850.1362166139152646No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC7710.1337872730301516No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA7590.13170498084291188No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT7490.12996973735354544No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA7470.12962268865567217No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7460.12944916430673553No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7450.12927563995779887No Hit
ATTACATACCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATT7290.12649925037481258No Hit
GATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCT7200.12493753123438281No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT7130.12372286079182632No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG7090.12302876339607974No Hit
CCTCCCGTGTACAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA6940.1204258981620301No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6730.1167818868343606No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6720.11660836248542396No Hit
GTGATTCGCAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGA6690.11608778943861403No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA6620.11487311899605754No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6600.11452607029818423No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGATCATTAACCTAATCTCCTCC6580.11417902160031096No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT6570.11400549725137431No Hit
ATGTAAGACCTTGGGCAAGCCCTGGCGGCCGTTCACGGTGGTCTCCATGC6470.11227025376200789No Hit
CCTCCCGCGTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6460.11209672941307125No Hit
GTATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAA6450.11192320506413458No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6430.11157615636626132No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6420.11140263201732468No Hit
CTTCATGGGGTATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATA6280.10897329113221167No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6260.1086262424343384No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6250.10845271808540173No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6230.10810566938752847No Hit
GCCCCATCCAGTGGGGGTTGCATCAGCAAACACTTGGCAGAGACCTGACC6200.10758509634071853No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6190.10741157199178188No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG6150.10671747459603531No Hit
GTACGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA6100.10584985285135211No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCATCAGCAAACACTTGGC6080.10550280415347882No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG6050.10498223110666889No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6000.10411460936198569No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA5960.1034205119662391No Hit
CCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCA5950.10324698761730246No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG5920.10272641457049253No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGGTATGTAA5810.10081764673218947No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5800.10064412238325281No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT5790.10047059803431617No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT5790.10047059803431617No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5780.10029707368537953No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5770.10012354933644288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)