FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576288
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33030.5731509245377311No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT23140.4015353434393914No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA20830.3614512188350269No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA20590.3572866344605475No Hit
CCCTTAACTTCATGGGGTATGTAATTGGAAGTTGGGGTACTTTACCACAG18380.3189377533455494No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC17600.30540285412849133No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATACCCCATGAA17560.3047087567327448No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT16220.2814564939752346No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA13770.23894302848575713No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC13050.22644927536231885No Hit
GTACACGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA12830.22263173968571268No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12380.21482314398356375No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA12120.21031151091121103No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT12020.20857626742184465No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA11950.20736159697928813No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA11510.19972652562607585No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC11490.19937947692820257No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC11260.1953884169026598No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG11230.19486784385584985No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA11040.19157088122605362No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG10730.1861916264090177No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10630.1844563829196513No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA10590.18376228552390472No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATACCCC10530.18272113943028487No Hit
GTCTGCACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT10520.1825476150813482No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA10470.181679993336665No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9820.17040091065578322No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9550.16571575323449386No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT9190.1594688766727747No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9030.15669248708978845No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAAT8880.1540896218557388No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG8850.15356904880892888No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT8830.15322200011105558No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT8830.15322200011105558No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATACCCCATGAAGTTAAGGGAG8780.1523543783663724No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8690.1507926592259426No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC8630.14975151313232274No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG8620.1495779887833861No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGT8610.14940446443444944No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATATGTT8600.1492309400855128No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8590.14905741573657616No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8570.14871036703870286No Hit
GATTTTTAGGCCCATATTAACATTGACATAGCTGACTACTAATTCCCTGG8280.14367816091954022No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8240.14298406352379367No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG8220.14263701482592037No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG8190.14211644177911043No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA8130.14107529568549057No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA8060.13986062524293408No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG8000.13881947914931422No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT7950.137951857404631No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA7850.1362166139152646No Hit
GATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCT7840.13604308956632793No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT7840.13604308956632793No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT7830.1358695652173913No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7740.1343078460769615No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT7730.13413432172802486No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7640.1325726025875951No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG7610.13205202954078515No Hit
CTTTACCACAGGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAA7580.1315314564939752No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT7540.13083735909822866No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT7440.12910211560886223No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7290.12649925037481258No Hit
ATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA7250.12580515297906603No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC7120.12354933644288967No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6950.12059942251096674No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6880.11938475206841025No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTT6870.11921122771947358No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6820.11834360597479038No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG6700.11626131378755067No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6700.11626131378755067No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6650.11539369204286747No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6640.1152201676939308No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA6600.11452607029818423No Hit
ATTACATACCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATT6560.11383197290243767No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6450.11192320506413458No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA6430.11157615636626132No Hit
CTTCATGGGGTATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATA6390.11088205897051476No Hit
GTGATTCGCAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGA6340.11001443722583153No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6320.10966738852795824No Hit
GTATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAA6270.10879976678327503No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC6250.10845271808540173No Hit
CCTCCCGCGTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6230.10810566938752847No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGATCATTAACCTAATCTCCTCC6130.10637042589816204No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG6120.10619690154922538No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT6110.10602337720028875No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT6100.10584985285135211No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6010.10428813371092233No Hit
GTACGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA6000.10411460936198569No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6000.10411460936198569No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT5990.10394108501304902No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5960.1034205119662391No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5950.10324698761730246No Hit
GTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT5910.10255289022155589No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT5810.10081764673218947No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5790.10047059803431617No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT5780.10029707368537953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)