FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378090
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA25870.6842286228146737No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT15490.40969081435637017No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC13700.362347589198339No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11590.30654077071596714No Hit
CATTTAAACCCTAACAAAACTAAACGATGGGGTTACTCTTTAAATTTCAT11330.29966410113994024No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11100.29358089343807026No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9970.26369382951149195No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9170.24253484620064006No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8970.23724510037292706No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC8940.23645163849877016No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8710.23036843079690023No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT8540.22587214684334417No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGACCTTGTAAGTTGGCGA8390.22190483747255943No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8350.22084688830701685No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8280.21899547726731727No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7760.20524213811526354No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC7650.2023327779100214No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7610.20127482874447883No Hit
ACTTTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATAT7440.1967785447909228No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7370.19492713375122325No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC7320.193604697294295No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7260.19201777354598112No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG7170.18963738792351026No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT7060.18672802771826813No Hit
CTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTTTACCTGTC6950.183818667513026No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6880.18196725647332646No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT6860.18143828189055516No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT6730.17799994710254172No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACC6590.17429712502314262No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6510.17218122669205746No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6490.17165225210928614No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6430.17006532836097227No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6360.16821391732127272No Hit
GTGGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG6290.16636250628157317No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG6280.16609801899018753No Hit
ATATTGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCC6160.16292417149355973No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC6150.16265968420217408No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6140.16239519691078844No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG6100.16133724774524585No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6010.158956862122775No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6010.158956862122775No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6000.15869237483138934No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA5920.15657647650030418No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5910.1563119892089185No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5790.15313814171229073No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC5790.15313814171229073No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG5670.14996429421566293No Hit
GTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGTTGCCTCCCTGACTGGCG5610.14837737046734906No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT5570.14731942130180645No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5460.14441006109656432No Hit
CAGTAAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTAT5430.14361659922240735No Hit
GTTAGTATTCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTC5380.14229416276547913No Hit
GGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG5360.1417651881827078No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5310.14044275172577958No Hit
ATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5290.13991377714300826No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5270.139384802560237No Hit
GAATACAGGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA5270.139384802560237No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA5250.13885582797746568No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5210.1377978788119231No Hit
TCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTT5100.13488851860668094No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC5070.134095056732524No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC5000.13224364569282446No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4960.13118569652728188No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4940.13065672194451058No Hit
TGTTTACACAGAAAGACCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4910.12986326007035362No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4900.12959877277896797No Hit
GCTTTATTCTTCTACTTTACCTGTCTTTAATCCTCATTGGAAAACACCCT4790.12668941257372582No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4770.12616043799095453No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA4690.12404453965986935No Hit
CAATAGGTCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT4680.12378005236848369No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG4670.12351556507709804No Hit
CAGAAAGACCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA4620.1221931286201698No Hit
ATACTGCACTCAGGCAAGCAATACTTTGCTGGGGGGAACTAATGACTCTA4590.12139966674601285No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4580.12113517945462722No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTGTCCGTCCCGAGAATGTTT4550.12034171758047027No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA4520.11954825570631332No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4510.11928376841492767No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG4430.11716787008384247No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4430.11716787008384247No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGAT4400.11637440820968553No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4330.11452299716998598No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA4270.11293607342167208No Hit
TTGTATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTA4270.11293607342167208No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT4230.1118781242561295No Hit
GACATAGCCCATGAAATTTAAAGAGTAACCCCATCGTTTAGTTTTGTTAG4230.1118781242561295No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4220.11161363696474386No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC4210.11134914967335818No Hit
CTCCGAAAGACACCAAATACTCTATGACTGTTTCTCTTCCAAAAGTGAGA4120.10896876405088736No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA4110.10870427675950171No Hit
TCACCATACTGCACTCAGGCAAGCAATACTTTGCTGGGGGGAACTAATGA4080.10791081488534476No Hit
TCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTT4070.1076463275939591No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4050.10711735301118781No Hit
GTAAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC4030.10658837842841651No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4010.10605940384564522No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA3990.10553042926287391No Hit
ATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCC3990.10553042926287391No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT3940.10420799280594567No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT3940.10420799280594567No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATTCAATCTAAGC3940.10420799280594567No Hit
TTGTAGGCCCACTCACAGTAAATGAGAAACGAAGACTGCAATTAATTATG3920.10367901822317437No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG3900.10315004364040309No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT3880.10262106905763177No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3860.10209209447486049No Hit
ATTAATTGCAGTCTTCGTTTCTCATTTACTGTGAGTGGGCCTACAAATTG3860.10209209447486049No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC3840.10156311989208919No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG3820.1010341453093179No Hit
TTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTCCTAAAAC3810.10076965801793224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)