FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378090
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA27190.7191409452775794No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC13180.34859425004628525No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12760.337485783808088No Hit
CATTTAAACCCTAACAAAACTAAACGATGGGGTTACTCTTTAAATTTCAT12160.32161654632494907No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11900.3147398767489222No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10790.2853817874051152No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC10390.27480229574968923No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10290.2721574228358328No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9940.262900367637335No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT9740.2576106218096221No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8810.23301330371075668No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG8660.22904599433997197No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8050.2129122695654474No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7880.20841598561189134No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7650.2023327779100214No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT7620.20153931603586447No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7610.20127482874447883No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7550.19968790499616496No Hit
ACTTTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATAT7170.18963738792351026No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGACCTTGTAAGTTGGCGA7120.18831495146658203No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7110.18805046417519639No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6850.1811737945991695No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6750.17852892168531304No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC6680.1766775106456135No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6600.17456161231452827No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6490.17165225210928614No Hit
ATATTGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCC6370.16847840461265837No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6360.16821391732127272No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT6340.1676849427385014No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC6240.16504006982464492No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA6230.16477558253325927No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6130.1621307096194028No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG5890.15578301462614721No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC5820.15393160358644767No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5720.15128673067259119No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5710.15102224338120554No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5700.1507577560898199No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT5670.14996429421566293No Hit
GTGGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG5640.149170832341506No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5630.14890634505012035No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5620.14864185775873467No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACC5580.1475839085931921No Hit
CTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTTTACCTGTC5530.14626147213626386No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5520.14599698484487822No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC5500.1454680102621069No Hit
GTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGTTGCCTCCCTGACTGGCG5400.14282313734825042No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5360.1417651881827078No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG5330.14097172630855087No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5270.139384802560237No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG5240.13859134068608003No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA5080.13435954402390965No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5030.1330371075669814No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5020.13277262027559578No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4990.1319791584014388No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA4950.13092120923589623No Hit
GAATACAGGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4940.13065672194451058No Hit
GGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG4930.1303922346531249No Hit
GTTAGTATTCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTC4890.12933428548758233No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC4890.12933428548758233No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4840.12801184903065407No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA4800.1269538998651115No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4800.1269538998651115No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT4780.12642492528234017No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA4780.12642492528234017No Hit
CAATAGGTCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT4730.12510248882541194No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC4700.124309026951255No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC4680.12378005236848369No Hit
CAGTAAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTAT4600.1216641540373985No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4590.12139966674601285No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4550.12034171758047027No Hit
TCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTT4550.12034171758047027No Hit
TTGTATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTA4540.1200772302890846No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4530.11981274299769895No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4490.11875479383215637No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC4460.11796133195799942No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGAT4420.11690338279245682No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT4360.11531645904414292No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4360.11531645904414292No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTTAC4340.11478748446137163No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4290.1134650480044434No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG4260.11267158613028644No Hit
TGTTTACACAGAAAGACCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4250.11240709883890078No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA4180.11055568779920126No Hit
CTCCGAAAGACACCAAATACTCTATGACTGTTTCTCTTCCAAAAGTGAGA4150.10976222592504431No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG4130.109233251342273No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGACCCACCGTG4130.109233251342273No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT4110.10870427675950171No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT4100.10843978946811605No Hit
ATTAATTGCAGTCTTCGTTTCTCATTTACTGTGAGTGGGCCTACAAATTG4100.10843978946811605No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTGTCCGTCCCGAGAATGTTT4100.10843978946811605No Hit
GGGGACCCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT4070.1076463275939591No Hit
GCTTTATTCTTCTACTTTACCTGTCTTTAATCCTCATTGGAAAACACCCT4070.1076463275939591No Hit
CTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA4060.10738184030257346No Hit
GGCATAATTAATTGCAGTCTTCGTTTCTCATTTACTGTGAGTGGGCCTAC4060.10738184030257346No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGAT4060.10738184030257346No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4030.10658837842841651No Hit
TCTCGGGGCCGCTTGGGGATCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG4030.10658837842841651No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4010.10605940384564522No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATTCAATCTAAGC4010.10605940384564522No Hit
ATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC3970.10500145468010262No Hit
ATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCC3960.10473696738871696No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAACGATGGGGTTACT3900.10315004364040309No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG3900.10315004364040309No Hit
ATACTGCACTCAGGCAAGCAATACTTTGCTGGGGGGAACTAATGACTCTA3880.10262106905763177No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG3870.10235658176624614No Hit
TTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTCCTAAAAC3810.10076965801793224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)