FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362723
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG19710.5433898594795478No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18680.5149935350115653No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18570.511960917835373No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17420.4802562837206353No Hit
CCTTTAAATGAATGTTGGGAAAGGAAGGAGTTTGCCACTCAGGATTAAAG15540.42842609925480324No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13320.36722237078983133No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA12540.34571835808592233No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG11420.3148408013828734No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT11060.3049158724426077No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10790.2974721757374085No Hit
CTTATATAAGAGCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA10400.2867201693854539No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10160.28010355009194343No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGT9250.25501553527071624No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9120.25143153315339806No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8520.23488998491962187No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8510.23461429244905893No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8440.23268444515511838No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8430.23240875268455544No Hit
CCTGTAAATGAATGTTGGGAAAGGAAGGAGTTTGCCACTCAGGATTAAAG8260.22772198068498553No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7950.21917551409753447No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7910.21807274421528272No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7870.21696997433303097No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7570.20869920021614288No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCCCTCAGGCA7400.204012428216573No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7330.20208258092263243No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTC7310.20153119598150657No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6940.1913305745706779No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6790.18719518751223385No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6770.18664380257110796No Hit
GGTTAGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCAGGACACG6550.1805785682187234No Hit
TCACCATACAGCCCTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA6250.17230779410183528No Hit
ATCCTAACTTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6110.16844809951395417No Hit
GTATAAAGAGTTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6060.1670696371611395No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAAGTT6050.16679394469057657No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6040.16651825222001362No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT5980.16486409739663602No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5950.16403701998494719No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT5950.16403701998494719No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5780.15935024798537728No Hit
GTGAAAGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAA5760.1587988630442514No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5700.15714470822087379No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5430.1497010115156745No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5400.1488739341039857No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5390.14859824163342275No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5370.1480468566922969No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACAATATGG5370.1480468566922969No Hit
GCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAAGGTTGTGGAGTTCC5360.14777116422173395No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCTTCCTTTCCCAAC5350.147495471751171No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5350.147495471751171No Hit
GTTCTATCCTAACTTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5250.14473854704554165No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5250.14473854704554165No Hit
AAAACTCAGACAAATATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA5240.1444628545749787No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5190.14308439222216401No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5150.14198162233991227No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA5120.14115454492822346No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5090.14032746751653466No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5040.13894900516371997No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5020.13839762022259408No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4970.13701915786977942No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4920.13564069551696473No Hit
AGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4890.13481361810527592No Hit
GGAAAATATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAAGGT4870.13426223316415006No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTGGGAAAGGAAGGAGTTTG4870.13426223316415006No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAGCTCTGCTAG4860.13398654069358712No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4820.13288377081133537No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4780.13178100092908362No Hit
GGAAAATATAGGCCTTTCACTCTGGGATCTAGCAGAGCTTGGTGGAAGGT4620.12736992140007664No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4610.12709422892951372No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4600.12681853645895078No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4510.12433730422388434No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA4440.12240745692994379No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC4440.12240745692994379No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4370.12047760963600322No Hit
CTTTAATCCTGAGTGGCAAACTCCTTCCTTTCCCAACATTCATTTACAGG4330.1193748397537515No Hit
GCCTATAGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4320.11909914728318856No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCTTCCTTTC4320.11909914728318856No Hit
TAGCCGGCATAATTAATTTTAATCTCCTTTTTTCATTAACTGTAAGAGGG4300.11854776234206268No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4290.11827206987149975No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATATGTT4280.11799637740093681No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4260.11744499245981094No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4250.11716929998924801No Hit
CTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG4220.11634222257755919No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4220.11634222257755919No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG4170.11496376022474451No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4150.11441237528361864No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4140.1141366828130557No Hit
CAATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTA4120.11358529787192982No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4110.11330960540136689No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4110.11330960540136689No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4100.11303391293080395No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4050.11165545057798927No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4030.1111040656368634No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4020.11082837316630045No Hit
GCTATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT4010.11055268069573752No Hit
GTTGCTGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTC3990.11000129575461165No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3940.10862283340179696No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3940.10862283340179696No Hit
ATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTACT3930.10834714093123403No Hit
TGCCTGCTAGGTTCTATCCTAACTTTACCAAATATTTGCCCTTGGATAAA3910.10779575599010816No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3880.10696867857841934No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3860.10641729363729346No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3840.10586590869616759No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT3800.10476313881391584No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC3770.10393606140222704No Hit
GCCCTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3770.10393606140222704No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3740.10310898399053825No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATA3730.1028332915199753No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3720.10255759904941238No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3700.1020062141082865No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3700.1020062141082865No Hit
GACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGG3690.10173052163772356No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT3670.10117913669659769No Hit
GCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCTCAGGCCATGC3660.10090344422603476No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC3660.10090344422603476No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3650.10062775175547181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)