FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416974
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA26350.6319338855660065No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC24460.5866073184419172No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA21900.5252126031838915No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA13620.32663907102121476No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA13410.32160278578520485No Hit
CCTTTCACACAATGTGGCTATCCTGCCTTGATGCCTTTGTATGCGTGTAT13400.32136296267872816No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT12920.3098514535678483No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12020.28826737398494867No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11950.2865886122396121No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11370.27267887206396557No Hit
GTGTAAAAGGCGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11360.27243904895748894No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT11140.2671629406150024No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA11100.2662036481890957No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11050.2650045326567124No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC10950.26260630159194576No Hit
CTCTTACAGTTAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG10620.2546921390782159No Hit
CATTTACAGGAGGACATTATTAATAGGTGTCAACAATATGTGGGCCCTCT10190.24437974549971941No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9740.2335877057082696No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT9120.2187186731067165No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG9030.21656026514842655No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA8900.21344256476422988No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG8630.20696734088936No Hit
TTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACAT8630.20696734088936No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8500.2038496405051634No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8480.20336999429221006No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8400.20145140944039674No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT8120.1947363624590502No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC7920.18993990032951694No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7360.17650980636682384No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7310.17531069083444054No Hit
GTATACACGCATACAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7310.17531069083444054No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6970.16715670521423398No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6930.16619741278832734No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6870.16475847414946734No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTGTATGCGTGTAT6870.16475847414946734No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6820.16355935861708404No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6600.15828325027459747No Hit
CGCATGCGCCGATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTC6540.15684431163573748No Hit
CGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6500.15588501920983083No Hit
GTACTAAAAATCAAGCACTGTTTTCGGAAACTGCCTGTAAATAGACCTAT6490.15564519610335417No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTTTTCA6390.15324696503858753No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6370.1527673188256342No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6240.1496496184414376No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGGTTAAAG6010.14413368699247434No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA6000.1438938638859977No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5930.14221510214066105No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5820.13957704796941775No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5710.13693899379817448No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT5650.1355000551593145No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT5630.13502040894636116No Hit
GTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA5620.1347805858398845No Hit
GACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGC5540.1328620009880712No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5350.1283053619650146No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC5300.1271062464326313No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA5260.12614695400672465No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG5260.12614695400672465No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5120.12278943051605136No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC5050.12111066877071473No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4970.11919208391890143No Hit
GCACACCCGAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAAC4960.11895226081242476No Hit
GTGTAAAAGGCGCAGCAAAGCCTAAAAGACCTACAATTCTTTGACATACT4860.11655402974765812No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4860.11655402974765812No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4810.11535491421527481No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4800.11511509110879814No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4740.11367615246993819No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4740.11367615246993819No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4720.11319650625698485No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG4670.11199739072460153No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4660.11175756761812487No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4660.11175756761812487No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4640.11127792140517155No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4640.11127792140517155No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4600.1103186289792649No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4520.10840004412745159No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4510.10816022102097492No Hit
CCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA4450.10672128238211494No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4450.10672128238211494No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA4440.10648145927563829No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4440.10648145927563829No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT4420.10600181306268497No Hit
GACCTGACCGTTGCCGTGCAACGGGGTAAAGGTGCAGATATTGTTTACAC4420.10600181306268497No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4410.1057619899562083No Hit
GCGCCGATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAA4400.10552216684973163No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4390.10528234374325499No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4380.10504252063677831No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4370.10480269753030166No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4370.10480269753030166No Hit
TTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA4330.103843405104395No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4310.10336375889144168No Hit
CGATAAGTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCGAGGAGT4280.10264428957201169No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG4270.10240446646553503No Hit
GGGTTCACTCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA4260.10216464335905835No Hit
AAGCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA4190.10048588161372173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)