FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416974
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA26020.6240197230522766No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20350.4880400216800088No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA14100.3381505801320946No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA13620.32663907102121476No Hit
CCTTTCACACAATGTGGCTATCCTGCCTTGATGCCTTTGTATGCGTGTAT13460.3228019013175881No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13290.31872490850748486No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12100.290185958836762No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11890.28514967360075205No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT11250.2698009947862457No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10720.2570903701429825No Hit
GTGTAAAAGGCGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10690.25637090082355257No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT10480.2513346155875426No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA10390.2491762076292527No Hit
CTCTTACAGTTAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG10260.246058507245056No Hit
CATTTACAGGAGGACATTATTAATAGGTGTCAACAATATGTGGGCCCTCT9830.23574611366655954No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG9180.22015761174557646No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA8940.21440185719013655No Hit
TTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACAT8850.21224344923184657No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8850.21224344923184657No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG8650.20744698710231332No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8590.20600804846345336No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT8440.20241070186630342No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8330.19977264769506015No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7960.1908991927554236No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA7840.18802131547770365No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7460.17890803743159045No Hit
GTACTAAAAATCAAGCACTGTTTTCGGAAACTGCCTGTAAATAGACCTAT7430.17818856811216047No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTGTATGCGTGTAT7220.17315228287615056No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7190.17243281355672058No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7040.1688354669595706No Hit
GTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA6810.16331953551060738No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGGTTAAAG6780.1626000661911774No Hit
GTATACACGCATACAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6760.16212041997822407No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6670.1599620120199341No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC6560.1573239578486908No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6440.15444608057097084No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6390.15324696503858753No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTTTTCA6360.15252749571915755No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6210.1489301491220076No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6080.145812448737811No Hit
CGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5980.14341421767304435No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5860.14053634039532442No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC5650.1355000551593145No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5570.1335814703075012No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5480.13142306234921122No Hit
CGCATGCGCCGATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTC5430.13022394681682792No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5280.12662660021967798No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT5280.12662660021967798No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5280.12662660021967798No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5270.1263867771132013No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT5260.12614695400672465No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5180.12422836915491134No Hit
GACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGC5180.12422836915491134No Hit
CCTTTACCCCGTTGCACGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5100.12230978430309804No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA5080.1218301380901447No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4980.11943190702537808No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT4960.11895226081242476No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4850.11631420664118147No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC4840.1160743835347048No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4800.11511509110879814No Hit
GCGCCTGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAAC4780.11463544489584482No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4760.11415579868289151No Hit
GTGTAAAAGGCGCAGCAAAGCCTAAAAGACCTACAATTCTTTGACATACT4750.11391597557641484No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4750.11391597557641484No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG4670.11199739072460153No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA4640.11127792140517155No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4610.11055845208574155No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4540.10887969034040493No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4530.10863986723392825No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4490.1076805748080216No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4480.10744075170154495No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA4440.10648145927563829No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG4410.1057619899562083No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4360.10456287442382499No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4330.103843405104395No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4310.10336375889144168No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4290.10288411267848835No Hit
TTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA4260.10216464335905835No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4250.1019248202525817No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4210.10096552782667505No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4190.10048588161372173No Hit
GGCCTCTACCGTCCCCTTCTTTCTCTGCCGTTCCGGCCGACCACGGGGCG4190.10048588161372173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)