FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784886
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT48720.6207270864813489No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG46950.5981760408517925No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG29850.38031000680353577No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC27060.3447634433535571No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA26480.33737383518116004No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA23850.3038657843304633No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT23810.30335615618064277No Hit
GTATACACGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA22640.28844953279839364No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA22470.28628361316165657No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT22040.2808051105510864No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA21850.27838437683943906No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT21210.2702303264423114No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20910.26640811531865777No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG20820.26526145198156165No Hit
GTATCCATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAAC20500.26118442678299775No Hit
GTGTGTAGTTTCCCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA19980.2545592608353315No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT17330.22079639590972447No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG17120.2181208481231669No Hit
CTCCAAAAGACACCAAATACTCAAGCACAGTTTCTCTTCCAAAAGTAAGA16570.2111134610631353No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC16320.20792828512675726No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15280.1946779532314247No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA15250.19429573211905934No Hit
GTGTAGTTTCCCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA15120.19263944063214275No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA15070.19200240544486716No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG14850.18919945062085447No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC14680.18703353098411743No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT14620.18626908875938672No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC14050.17900688762444483No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG13910.17722318910007312No Hit
ATCCTAACCTTACCAAATATTTGCCATTGGATAAAGGCATTAAACCTTAT13820.17607652576297705No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC13650.17391060612623999No Hit
CATTCCTTTAGTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACT13500.17199950056441318No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC13500.17199950056441318No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13390.17059802315240685No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG13360.1702158020400415No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG13280.16919654574040052No Hit
CGTGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC13260.16894173166549029No Hit
CTATATAAGAGGGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA13230.1685595105531249No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA12970.16524692757929177No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG12760.16257137979273423No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG12700.1618069375680035No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT12600.1605328671934523No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG12570.16015064608108695No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCGTGTATA12460.15874916866908062No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12400.15798472644434988No Hit
ATGTAAGACCTTGGGCAACACCTGGTGGGCGTTCACGGTGGTCTCCATGC12330.15709287718216403No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12310.1568380631072538No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC12020.15314325902105527No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11990.15276103790868992No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA11900.15161437457159385No Hit
AAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTGATTGGAAAG11850.15097733938431823No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC11770.14995808308467726No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT11730.14944845493485678No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC11570.14740994233557483No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG11520.14677290714829924No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA11010.14027514823808807No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT10890.13874626378862662No Hit
TCCCGATACAGAGCAGAGGCGGTGTCAAGGAGATCTCGAATAGAAGGAAA10880.13861885675117153No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC10700.1363255300769793No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG10690.13619812303952422No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC10670.13594330896461396No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC10640.1355610878522486No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC10390.1323759119158706No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC10060.1281714796798516No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT9990.12727963041766574No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGT9940.12664259523039015No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA9900.12613296708056967No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG9880.1258781530056594No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT9830.12524111781838382No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAAT9810.12498630374347358No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATATGTT9790.12473148966856333No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA9640.12282038410673653No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATA9630.1226929770692814No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT9610.12243816299437116No Hit
GCTTAGATTGTATACACGCATATAAAGGCATCAAAGCAGGATAGCCACAT9560.12180112780709555No Hit
GTGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC9540.1215463137321853No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT9480.12078187150745459No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9450.12039965039508922No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT9370.11938039409544825No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG9280.11823373075835217No Hit
CTAGTGGACCTGCCTCGACATCTAACAACAGTAGTTTCCGGAAGTGTTGA9260.11797891668344192No Hit
CTTTATATGCGTGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT9170.11683225334634584No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG9150.11657743927143559No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT8950.11402929852233318No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC8810.11224559999796148No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA8730.1112263436983205No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8730.1112263436983205No Hit
CCCTCATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCATGGGATATG8710.11097152962341028No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG8480.1080411677619425No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC8470.10791376072448737No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT8290.1056204340502952No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8240.10498339886301959No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT8240.10498339886301959No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT8160.10396414256337863No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCAAGGAGATCTCGAA8140.1037093284884684No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC8110.10332710737610304No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT8010.10205303700155181No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGCA7970.10154340885173134No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC7950.10128859477682109No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7910.1007789666270006No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG7870.10026933847718013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)