FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784886
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG52950.674620263324865No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT46200.5886205130426584No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT28520.36336487082200475No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG28440.36234561452236375No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC25810.32883756367166694No Hit
GTATACACGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA25760.3282005284843914No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA25420.32386868921091727No Hit
GTGTGTAGTTTCCCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA25310.3224672117989109No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA24710.3148227895516037No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT21780.27749252757725323No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA21020.26780959273066407No Hit
GTATCCATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAAC20790.2648792308691963No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA20440.26041998455826704No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT20420.2601651704833568No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC19290.24576817525092817No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19040.24258299931455013No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG18940.24130892893999895No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT18690.2381237530036209No Hit
GTGTAGTTTCCCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA17750.22614749148283955No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT17670.22512823518319858No Hit
CTCCAAAAGACACCAAATACTCAAGCACAGTTTCTCTTCCAAAAGTAAGA17220.21939491849771814No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA16470.20983939068858407No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG16430.2093297625387636No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG16220.20665421475220608No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16020.20410607400310363No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT15410.19633424471834127No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14960.19060092803286083No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG14780.18830760135866864No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG14760.1880527872837584No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG14510.1848676113473804No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC14440.18397576208519453No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC14080.1793891087368102No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA13510.1721269076018683No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG13190.16804988240330443No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC13120.16715803314111857No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA12990.165501741654202No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG12820.16333582201746494No Hit
CATTCCTTTAGTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACT12800.16308100794255473No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12420.15823954051926012No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCGTGTATA12410.158112133481805No Hit
CTATATAAGAGGGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA12410.158112133481805No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG11890.15148696753413873No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC11880.1513595604966836No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11710.14919364085994655No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA11510.14664550011084412No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG11480.14626327899847877No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC11360.14473439454901732No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT11260.1434603241744661No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC11190.14256847491228025No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC11160.1421862537999149No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATA10880.13861885675117153No Hit
AAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTGATTGGAAAG10870.13849144971371638No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC10860.13836404267626126No Hit
ATCCTAACCTTACCAAATATTTGCCATTGGATAAAGGCATTAAACCTTAT10840.13810922860135103No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC10790.13747219341407543No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA10730.1367077511893447No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT10610.13517886673988325No Hit
CGTGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10510.13390479636533204No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10270.13084702746640914No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG10170.12957295709185793No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT10160.1294455500544028No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG10090.12855370079221695No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC10060.1281714796798516No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG9900.12613296708056967No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9840.12536852485583894No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT9670.12320260521910188No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9620.12256557003182628No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG9540.1215463137321853No Hit
CCCTCATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCATGGGATATG9540.1215463137321853No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT9530.1214189066947302No Hit
CTTTATATGCGTGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT9510.12116409261981995No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT9370.11938039409544825No Hit
GCTTAGATTGTATACACGCATATAAAGGCATCAAAGCAGGATAGCCACAT9280.11823373075835217No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAAT9230.11759669557107656No Hit
GTCTTTGGGTATCCATTTGAACCCTAATAAAACCAAACGTTGGGGTTACT9060.11543077593433951No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC8980.11441151963469853No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT8940.11390189148487806No Hit
GTGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC8880.11313744926014734No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATATGTT8870.1130100422226922No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT8870.1130100422226922No Hit
ATGTAAGACCTTGGGCAACACCTGGTGGGCGTTCACGGTGGTCTCCATGC8860.11288263518523708No Hit
TCCCGATACAGAGCAGAGGCGGTGTCAAGGAGATCTCGAATAGAAGGAAA8860.11288263518523708No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT8750.11148115777323075No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC8690.11071671554850003No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC8500.10829598183685274No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8470.10791376072448737No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCAAGGAGATCTCGAA8450.10765894664957715No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG8400.10702191146230154No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC8330.10613006220011569No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT8330.10613006220011569No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG8300.10574784108775032No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC8300.10574784108775032No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC8290.1056204340502952No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG8250.10511080590047471No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC8240.10498339886301959No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA8230.10485599182556449No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC8190.10434636367574399No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG8170.10409154960083376No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT8130.10358192145101326No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGT8130.10358192145101326No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8120.10345451441355816No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATCCATTTGAA8120.10345451441355816No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA8110.10332710737610304No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8090.10307229330119279No Hit
CTAGTGGACCTGCCTCGACATCTAACAACAGTAGTTTCCGGAAGTGTTGA7890.10052415255209038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)