FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596020
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45210.7585315928995672No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG27220.456696084024026No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA23300.39092647897721555No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT22850.38337639676520924No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAA21360.35837723566323276No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA17990.3018355088755411No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT17860.29965437401429484No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16520.27717190698298716No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13380.22448911110365424No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA13380.22448911110365424No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG13200.2214690782188517No Hit
CTATTAATAATGTCCTCCCGTAAATGAATGTTAGGAAAGGAGGGAGTTTG13190.22130129861414044No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT12270.20586557498070537No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12200.20469111774772658No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11590.19445656186034024No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTAAC11400.19126874937082647No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA11350.19042985134727022No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10850.18204087111170766No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCA10730.18002751585517265No Hit
ATGTTAATATGGGCCTAAAATTAAGACAAATATTGTGGTTTCACATTTCC10650.17868527901748263No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG10070.16895406194423007No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT9490.1592228448709775No Hit
GGAACATATTGTACAAAAAATTAAGCAATGTTTTCGGAAACTGCCTGTAA9090.15251166068252742No Hit
CTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTC9010.15116942384483742No Hit
GCAAATCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGG9010.15116942384483742No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATGTCCTCCCGT8560.1436193416328311No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8540.1432837824234086No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTAATTTTTTGTACAATATGTT8430.14143820677158483No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8420.1412704271668736No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8350.14009596993389484No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8330.13976041072447232No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA8290.13908929230562733No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8240.1382503942820711No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8190.13741149625851481No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTG8040.13489480218784605No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA8030.1347270225831348No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7910.13271366732659978No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7750.13002919365121976No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC7720.129525854837086No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7720.129525854837086No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCA7620.1278480587899735No Hit
ACATATTGTTGACATCTATTAATAATGTCCTCCCGTAAATGAATGTTAGG7520.12617026274286097No Hit
TATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA7350.12331800946276972No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA7350.12331800946276972No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG7220.12113687460152343No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7170.12029797657796718No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCCAAACTAGGCATTATT7120.11945907855441093No Hit
GTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCTTTGACATACT7060.11845240092614343No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7000.11744572329787592No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6950.11660682527431965No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6820.11442569041307338No Hit
CTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCT6810.11425791080836215No Hit
CCCATGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTG6790.11392235159893964No Hit
CTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTAACATTCATTTACGGG6680.11207677594711586No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6620.11107009831884836No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA6610.11090231871413712No Hit
GCTTAGCTTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACAT6580.11039897990000336No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAATTTTTTGT6470.10855340424817958No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTAATTTTTTGTACAATA6380.10704338780577834No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA6280.10536559175866583No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6150.10318445689741955No Hit
ATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAG6150.10318445689741955No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6070.10184222005972954No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6070.10184222005972954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)