FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595730
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN89591.5038692024910614No Hit
TTATTAATAGATGTCAACAGTATGTGGGACCTCTTACAGTTAATGAAAAA31670.5316166719822738No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT31320.5257415272019204No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG29210.4903227972403606No Hit
AATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGG28040.47068302754603597No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG26050.4372786329377402No Hit
TTATTGATAGATGTCAACAGTATGTGGGACCTCTTACAGTTAATGAAAAA21720.3645946989407953No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT20640.34646568076141876No Hit
GTAAACGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA18690.31373273127087775No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA16940.2843570073691101No Hit
TATTAATAGATGTCAACAGTATGTGGGACCTCTTACAGTTAATGAAAAAA16420.27562822083829924No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG15790.26505296023366287No Hit
AACCCTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCAT13880.23299145586087658No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13590.2281234787571551No Hit
TATTGATAGATGTCAACAGTATGTGGGACCTCTTACAGTTAATGAAAAAA12810.21503029896093867No Hit
GATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGG12010.20160139660584495No Hit
GTAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA11730.19690128078156213No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11550.193879777751666No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11090.18615815889748713No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTGTC11000.18464740738253907No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA10970.18414382354422304No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10840.18196162691152032No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10530.17675792724892148No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT10170.1707149211891293No Hit
GGATAATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT10140.17021133735081329No Hit
GTATGTGGGACCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTA10100.1695398922330586No Hit
ATATTCTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA9990.1676934181592332No Hit
GGTTAGGGTAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9970.16735769560035585No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9870.16567908280596916No Hit
GTGTAAAAGGGGCTGCAAAGCCCAACAGACCCACAATTCTTTGACATACT9700.16282544105551172No Hit
CCCGTTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC9560.16047538314337031No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9470.15896463162842225No Hit
ATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGA9190.15426451580413947No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8920.1497322612592953No Hit
ACCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCCTAT8850.14855723230322462No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC8790.1475500646265926No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8780.1473822033471539No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTT8710.14620717439108322No Hit
ATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAG8380.14066775216960703No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8360.14033202961072969No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7840.13160324307991875No Hit
GTTTTACCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7780.13059607540328672No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA7660.12858174005002265No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7470.12539237574068787No Hit
ATTGATAGATGTCAACAGTATGTGGGACCTCTTACAGTTAATGAAAAAAG7460.12522451446124921No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7420.12455306934349454No Hit
ACCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCATAT7350.12337804038742384No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7320.12287445654910782No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATATGTT7230.12136370503415977No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGGTAAAAC7220.12119584375472109No Hit
GAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAA7150.1200208147986504No Hit
GGGTAAAGGTGCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7010.11767075688650899No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6990.11733503432763165No Hit
ACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGG6910.11599214409212227No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6830.1146492538566129No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAAT6820.11448139257717423No Hit
GGAGAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT6810.11431353129773555No Hit
TGCTTGATCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAACT6560.11011699931176876No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6530.10961341547345274No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6500.10910983163513671No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6340.10642405116411796No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6330.1062561898846793No Hit
CTGGCTGGGGCTTGGCTGTAGGCCATCAGCGCATGCGTGGAACCTTTGTG6280.10541688348748594No Hit
ATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGGT6250.10491329964916991No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGT6230.10457757709029258No Hit
CTATATAAGAGAGAAACAACACGCAGCGCTTCATTCTGTGGGTCACCATA6210.10424185453141524No Hit
AGACTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTT6140.10306682557534454No Hit
CATGTATACATGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC6120.10273110301646719No Hit
TCCCCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAA6040.10138821278095783No Hit
GGGCACATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATC6030.10122035150151915No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5970.10021318382488711No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)