FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595730
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG33570.5635103150756215No Hit
TTATTAATAGATGTCAACAGTATGTGGGACCTCTTACAGTTAATGAAAAA29920.5022409480805062No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT28960.4861262652543938No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG27700.4649757440451211No Hit
AATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGG26670.4476860322629379No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24280.40756718647709533No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT22230.3731556241921676No Hit
TTATTGATAGATGTCAACAGTATGTGGGACCTCTTACAGTTAATGAAAAA22120.3713091501183422No Hit
GTAAACGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA21070.35368371577728164No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG17170.2882178167961996No Hit
TATTAATAGATGTCAACAGTATGTGGGACCTCTTACAGTTAATGAAAAAA16090.27008879861682306No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA14720.247091803333725No Hit
GTAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA14560.24440602286270627No Hit
AACCCTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCAT13880.23299145586087658No Hit
GTGTAAAAGGGGCTGCAAAGCCCAACAGACCCACAATTCTTTGACATACT13730.2304735366692965No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12640.21217665721048126No Hit
TATTGATAGATGTCAACAGTATGTGGGACCTCTTACAGTTAATGAAAAAA12090.20294428684135432No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11790.19790844845819414No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11600.19471908414885938No Hit
GATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGG10970.18414382354422304No Hit
GTATGTGGGACCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTA10820.18162590435264298No Hit
ATATTCTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA10680.17927584644050157No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTGTC10560.1772615110872375No Hit
GGATAATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT10410.17474359189565744No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT10380.1742400080573414No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10010.16802914071811054No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9980.16752555687979453No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA9840.16517549896765313No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9830.16500763768821444No Hit
ATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGA9830.16500763768821444No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9800.16450405384989844No Hit
GGTTAGGGTAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9790.16433619257045978No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9590.16097896698168634No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA9440.15846104779010628No Hit
CCCGTTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC9040.15174659661255938No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA8960.15040370637705No Hit
ATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAG8740.14671075822939922No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8620.14469642287613516No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8380.14066775216960703No Hit
GTTTTACCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8310.1394927232135363No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC8240.13831769425746562No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA8230.13814983297802697No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGGTAAAAC8120.13630335890420156No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTT7990.13412116227149884No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7940.13328185587430547No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATATGTT7930.1331139945948668No Hit
GGGTAAAGGTGCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7870.13210682691823478No Hit
ACCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCCTAT7750.1300924915649707No Hit
ATTGATAGATGTCAACAGTATGTGGGACCTCTTACAGTTAATGAAAAAAG7690.12908532388833868No Hit
GAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAA7560.12690312725563593No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7300.12253873399023048No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7240.12153156631359845No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6930.1163278666509996No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAAT6850.11498497641549024No Hit
CTATATAAGAGAGAAACAACACGCAGCGCTTCATTCTGTGGGTCACCATA6820.11448139257717423No Hit
ATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGGT6750.11330636362110351No Hit
ACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGG6670.11196347338559415No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6640.11145988954727813No Hit
ACCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCATAT6580.1104527218706461No Hit
GGAGAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT6350.10659191244355665No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6300.10575260604636329No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGT6240.10474543836973126No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6110.10256324173702851No Hit
TGCTTGATCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAACT6090.10222751917815116No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6020.10105249022208046No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6010.1008846289426418No Hit
AGACTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTT6010.1008846289426418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)