FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480557
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28710.5974317302630072No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG26980.5614318384707746No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16690.3473053144580143No Hit
TTATTAATAGATGCCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA16230.33773308889476167No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG15070.3135944331265594No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13070.27197606111241746No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT11570.24076228210181103No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGA10260.21350224843254806No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9570.1991439100876691No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9020.18769885778378007No Hit
GTTTTAGTACAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACA8970.18665839848342652No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8720.18145610198165876No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8500.17687808106010317No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC8090.16834631479720408No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8030.16709776363677983No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA7860.16356020201557775No Hit
GTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT7850.16335211015550705No Hit
ATTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7790.16210355899508277No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7730.16085500783465853No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACACACT7510.1562769869131029No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT7330.15253133343183015No Hit
GTTTTTGTACAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACA7310.1521151497116887No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7310.1521151497116887No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7250.15086659855126447No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7150.1487856799505574No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7130.14836949623041595No Hit
TATTAATAGATGCCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA7070.1471209450699917No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG6980.1452481183293553No Hit
CTGTAAGAGGGCCCACATATTGTTGGCATCTATTAATAATGTCCTCCTGT6640.1381729950869512No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6620.13775681136680978No Hit
GATTAAAATTAATTATGCCTGCTAGATTTTATCCTAACCTTACCAAATAT6620.13775681136680978No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6480.13484352532581984No Hit
CACATATTGTTGGCATCTATTAATAATGTCCTCCTGTAAATGAATGTTAG6420.1335949741653956No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6350.13213833114490062No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6280.13068168812440564No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG6150.12797649394348642No Hit
GGTTAGGATAAAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5980.12443893232228435No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5950.12381465674207223No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5940.1236065648820015No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5880.12235801372157726No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5700.11861236024030448No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC5690.11840426838023377No Hit
GTACAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5650.11757190093995093No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5640.11736380907988021No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5500.11445052303889029No Hit
ATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCC5160.10737539979648615No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT5140.10695921607634473No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5140.10695921607634473No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC5110.10633494049613261No Hit
GATTAAGATTAATTATGCCTGCTAGATTTTATCCTAACCTTACCAAATAT5090.10591875677599119No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG5070.10550257305584979No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGACGTTGACATTGCTGAGAGTCCAA5010.10425402189542551No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4990.1038378381752841No Hit
CCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACCGAGT4920.10238119515478913No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT4870.10134073585443559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)