FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577817
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG55010.9520315255522077No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA37000.6403411460721993No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT36490.6315148221668798No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG28530.49375494317404994No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG26970.4667567759342491No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA24520.42435580815379265No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT21750.3764167547856847No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21050.3643021925626972No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT19220.33263126560831546No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA17940.3104789232577096No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT17490.30269099040007474No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA17480.30251792522546067No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT16340.2827884953194523No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA16200.28036558287485486No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG14740.25509806738119506No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14710.2545788718573528No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA14360.248521590745859No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG14330.24800239522201664No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG14190.24557948277741914No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA13970.24177204893590878No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG13620.235714767824415No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC13090.22654231356986726No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12430.21512001204533615No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12100.2094088612830706No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT12090.20923579610845647No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC11560.20006334185390875No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA11470.19850575528238182No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11320.19590977766317016No Hit
GATTAAGATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT11080.19175621347243157No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG11030.19089088759936104No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11020.19071782242474694No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA10890.18846797515476355No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA10880.18829490998014942No Hit
TATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA10830.18742958410707888No Hit
CTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATA10790.18673732340862245No Hit
GGCAAAGACCTGCCCGTTGCCGGGCAACGGGGTAAAGGTGCAGATATTGT10760.18621812788478012No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC10420.18033391194790047No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA10330.17877632537637347No Hit
CTTTACCACAGGAACATATTGTACAAAAAATCAAGCAATGTTTTCGGAAA10030.17358437013795025No Hit
TTGACATACTTTCCAATCAATAGGTCTATTGACAGGCAGTTTCCGAAAAC9960.1723729139156515No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9860.17064226216951042No Hit
GGAACATATTGTACAAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTCA9830.1701230666456681No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA9590.1659695024549295No Hit
GATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCC9460.1637196551849461No Hit
CAATAGGTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGT9220.15956609099420752No Hit
ATCTCAATCTCGGGAACCTCAATGTTAGTATTCCTTGGACTCATAAGGTG9100.15748930889883822No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC9080.15714317854961No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG9040.15645091785115356No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC9020.15610478750192536No Hit
CCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACCGAGT8810.1524704188350291No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8770.15177815813657267No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8700.15056670191427388No Hit
ATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG8540.14779765912044815No Hit
GTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGTACAATA8470.14658620289814941No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8460.1464131377235353No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGACGTTGACATTGCTGGGAGTCCAA8350.14450942080278012No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT8240.14260570388202495No Hit
AATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCCTTGG8110.14035585661204153No Hit
CCCATAAAATGAAGCGCTGCGTGTAGTTTCTCTCTTATATAGAATGCCAG8040.13914440038974277No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8040.13914440038974277No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA7990.13827907451667223No Hit
GGATAAAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCATTAAC7910.13689455311975937No Hit
CCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTCTTATATAGAATGCCAG7890.13654842277053117No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG7780.13464470584977598No Hit
ATGTAAGACCTTGGGCAAGGCCTGATGGGCGTTCACGGTGGTCTCCATGC7770.13447164067516187No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC7720.1336063148020913No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA7690.133087119278249No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7620.13187566305595022No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7600.13152953270672202No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG7520.13014501130980916No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGGTT7500.12979888096058095No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7500.12979888096058095No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG7410.12824129438905396No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7390.12789516403982576No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7370.12754903369059753No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7270.12581838194445646No Hit
GACAAGAAATGTGAAACCACAATAGTTGTCTGATTTTTAGGCCCATATTA7160.12391466502370127No Hit
GGTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGTACAAT7050.12201094810294609No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6900.11941497048373448No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6870.11889577495989213No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6830.1182035142614357No Hit
TTTTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT6770.11716512321375107No Hit
TTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCTTGAGTATTTGGTGTCT6730.11647286251529464No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6720.11629979734068052No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT6680.11560753664222409No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG6630.11474221076915356No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6620.11456914559453944No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT6530.11301155902301249No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6330.1095502555307303No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG6240.10799266895920334No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6160.10660814756229048No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6150.10643508238767638No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC6040.10453136546692118No Hit
CTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT6010.10401216994307887No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG5990.10366603959385064No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT5950.1029737788953942No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA5950.1029737788953942No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5920.10245458337155189No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC5920.10245458337155189No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT5850.10124312714925314No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC5840.10107006197463902No Hit
ATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAG5830.10089699680002491No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGTCCGACCT5810.10055086645079671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)