FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577817
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG60241.0425446118753863No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT33240.5752686404172948No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA32820.5679999030835022No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG25760.44581588980594206No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG25390.43941247834522No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA23310.40341492202548557No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20500.3547836079589212No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT19900.3443996974820748No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT19730.341457589513635No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT18110.31342103122614945No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT17620.3049408376700582No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA17590.3044216421462158No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA17060.29524918789166815No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA16400.283826886367137No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14640.25336741563505405No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA14360.248521590745859No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG14270.246964004174332No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG14170.24523335242819091No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG14140.24471415690434864No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC13510.2338110509036598No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG13480.23329185537981748No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA13130.22723457426832372No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA13120.22706150909370962No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11840.20490916674310378No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC11610.20092866772697932No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11500.19902495080622412No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC11140.19279460452011624No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA11080.19175621347243157No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10910.18881410550399175No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA10880.18829490998014942No Hit
CTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATA10580.18310295474172616No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10420.18033391194790047No Hit
CTTTACCACAGGAACATATTGTACAAAAAATCAAGCAATGTTTTCGGAAA10400.17998778159867224No Hit
GATTAAGATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT10310.17843019502714527No Hit
GATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCC10280.17791099950330297No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10240.17721873880484654No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA10010.17323823978872202No Hit
GTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGTACAATA9630.16666176315338593No Hit
GGAACATATTGTACAAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTCA9540.16510417658185894No Hit
GGCAAAGACCTGCCCGTTGCCGGGCAACGGGGTAAAGGTGCAGATATTGT9520.16475804623263074No Hit
TATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA9480.1640657855341743No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG9340.1616428730895768No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA9330.16146980791496268No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9230.15973915616882162No Hit
TTGACATACTTTCCAATCAATAGGTCTATTGACAGGCAGTTTCCGAAAAC9170.15870076512113696No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC9040.15645091785115356No Hit
CAATAGGTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGT8800.15229735366041497No Hit
ATCTCAATCTCGGGAACCTCAATGTTAGTATTCCTTGGACTCATAAGGTG8680.15022057156504567No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC8450.14624007254892119No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA8420.14572087702507888No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGACGTTGACATTGCTGGGAGTCCAA8320.14399022527893782No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8280.14329796458048139No Hit
CCCATAAAATGAAGCGCTGCGTGTAGTTTCTCTCTTATATAGAATGCCAG8270.14312489940586726No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8260.14295183423125316No Hit
ATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG8210.1420865083581826No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8210.1420865083581826No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT8190.1417403780089544No Hit
CCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACCGAGT8060.139490530738971No Hit
TTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCTTGAGTATTTGGTGTCT7750.13412551032593364No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7640.13222179340517845No Hit
GGTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGTACAAT7520.13014501130980916No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7510.12997194613519505No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7510.12997194613519505No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7470.12927968543673862No Hit
AATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCCTTGG7440.1287604899128963No Hit
GGATAAAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCATTAAC7390.12789516403982576No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG7360.12737596851598343No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC7320.12668370781752702No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG7310.1265106426429129No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7250.12547225159522823No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7180.1242607953729295No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC7140.12356853467447307No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7060.1221840132775602No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7050.12201094810294609No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6950.120280296356805No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT6840.11837657943604982No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGGTT6810.1178573839122075No Hit
TTTTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT6710.11612673216606642No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG6710.11612673216606642No Hit
CCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTCTTATATAGAATGCCAG6690.1157806018168382No Hit
ATGTAAGACCTTGGGCAAGGCCTGATGGGCGTTCACGGTGGTCTCCATGC6650.11508834111838177No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG6630.11474221076915356No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA6580.11387688489608301No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGTCCGACCT6540.11318462419762658No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6410.11093477692764317No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT6400.11076171175302908No Hit
GACAAGAAATGTGAAACCACAATAGTTGTCTGATTTTTAGGCCCATATTA6370.11024251622918674No Hit
ATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAG6350.10989638587995854No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC6210.10747347343536102No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA6210.10747347343536102No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA6070.10505056099076353No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6040.10453136546692118No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6040.10453136546692118No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5880.10176232267309546No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5870.10158925749848134No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT5850.10124312714925314No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT5830.10089699680002491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)