FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429326
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20130.46887446835272034No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15540.3619627043319063No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13840.32236575469456774No Hit
TTATTAATCGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA11480.26739587166861545No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11390.2652995625701681No Hit
GTGTAAAAGGGGCAGCAAAGCCAAGAAGACCCACAATTCTTTGACATACT10600.24689862715046376No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10170.23688292812454873No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9290.21638568360639704No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9130.21265891187582397No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8280.1928604370571547No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8120.18913366532658166No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8030.18703735622813433No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCGATTAATAATGTCCTCCTGT7760.18074842893279233No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7740.1802825824664707No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG7730.18004965923330987No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7600.1770216572022193No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7290.16980103697423402No Hit
CATGTATACAAGCTAAGCAGGCTTTTACTTTTTCGCCAACTTACAAGGCC7060.1644438026115353No Hit
TTATCAGGGTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAATAGA7030.16374503291205286No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6930.16141580058044472No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6780.15792195208303247No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6770.15768902884987165No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6760.15745610561671083No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6740.1569902591503892No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6740.1569902591503892No Hit
GTGCTGGTGCACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6620.15419518035245944No Hit
GTACTAAAGCTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT6520.1518659480208513No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6520.1518659480208513No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTCTGTC6380.1486050227565999No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6350.14790625305711744No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6340.14767332982395662No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6330.1474404065907958No Hit
CCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATG6290.14650871365815255No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6280.14627579042499173No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6250.1455770207255093No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6200.14441240455970522No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6180.14394655809338358No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6140.14301486516074033No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAATAGA6140.14301486516074033No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6120.1425490186944187No Hit
AACACATTGTACTAAAGCTCAAGCAATGTTTTCGGAAACTGCCTGTAAAT6050.14091855606229298No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5990.1395210166633281No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5990.1395210166633281No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5860.1364930146322375No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5800.13509547523327262No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5710.1329991661348253No Hit
GATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAATTGGAGGAC5640.13136870350269958No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5540.12903947117109144No Hit
GATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGC5480.12764193177212654No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5470.12740900853896572No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5450.1269431620726441No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG5450.1269431620726441No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5420.12624439237316165No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5320.1239151600415535No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5250.1222846974094278No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5180.1206542347773021No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5170.12042131154414129No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4780.1113373054508695No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCAAGAAGACCCACAATTCT4770.1111043822177087No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC4760.11087145898454787No Hit
GTAGAGTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4740.11040561251822625No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4740.11040561251822625No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4730.11017268928506542No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAAT4720.10993976605190461No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4670.10877514988610053No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4620.10761053372029647No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4550.10598007108817076No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4530.10551422462184913No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC4520.10528130138868831No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4500.10481545492236669No Hit
GCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTT4470.10411668522288424No Hit
ATTGACAACAGTGCCAGCATCGCCTCCTCCTGCCTCCACCAATCGGCAGT4450.10365083875656261No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4400.10248622259075854No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4390.10225329935759772No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4390.10225329935759772No Hit
GCCAAGAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT4330.10085575995863283No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4320.10062283672547202No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4320.10062283672547202No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4300.10015699025915038No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4300.10015699025915038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)