FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421916
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16480.39059907659344517No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA13720.32518321182415455No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA13510.3202059177656216No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCAGG13050.3093032736374065No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG12030.2851278453531034No Hit
ATATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC11060.2621374870827369No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10970.2600043610576513No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT10960.259767347054864No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC10220.24222831084860494No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9800.23227372273153896No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9610.22777045667858056No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9590.227296428673006No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9190.2178158685615146No Hit
CCTAGTAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTC9160.21710482655315275No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9130.2163937845447909No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9130.2163937845447909No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9020.21378663051413077No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG8500.20146190236919195No Hit
AGTTTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAA8440.20003981835246826No Hit
TGGAAACCACCGTGAACGCCCACCAGGTCTTGCCCAAGCTCTTACATAAG8230.19506252429393528No Hit
CCTAGTTTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTT8070.19127030024933875No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7930.18795210421031674No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7920.18771509020752947No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7840.1858189781852312No Hit
TGTTCATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCCT7790.18463390817129477No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7710.18273779614899646No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7480.17728647408488893No Hit
CCTTTCTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG7480.17728647408488893No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7450.1765754320765271No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCTTCCTGT7180.1701760540012704No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7020.16638382995667383No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6960.1649617459399501No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC6880.16306563391765186No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6840.16211757790650272No Hit
AGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTT6790.1609325078925663No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6740.15974743787862988No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT6710.15903639587026802No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6700.15879938186748072No Hit
TGGATAAAGGCATTAAACCTTATTATCCTGAACATGTAGTTAATCATTAC6700.15879938186748072No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6440.15263701779501135No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA6380.15121493377828762No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6340.15026687776713848No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6120.14505256970581823No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6070.14386749969188178No Hit
CCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCT5960.14126034566122167No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5870.1391272196361361No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5850.13865319163056153No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5830.13817916362498697No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5830.13817916362498697No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5790.13723110761383783No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5770.13675707960826325No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5730.1358090235971141No Hit
GCCCAAAAGACCCACAATTCTTTGACAGACTTTCCAATCAATAGGTCTAT5720.13557200959432683No Hit
ATGTTCATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCC5700.13509798158875228No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG5670.1343869395803904No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5520.13083172953858113No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5370.12727651949677188No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5340.12656547748841002No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5300.12561742147726088No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5180.12277325344381346No Hit
ATGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCC5110.12111415542430247No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA5080.12040311341594062No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5050.11969207140757876No Hit
CTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5030.11921804340200419No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5020.11898102939921691No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA4990.11826998739085505No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4940.11708491737691865No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4900.11613686136576948No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4860.11518880535462035No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTTTCTCCTCC4860.11518880535462035No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4850.11495179135183307No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA4840.11471477734904577No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4820.11424074934347121No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG4810.11400373534068393No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4770.11305567932953478No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4670.11068553930166193No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4630.1097374832905128No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4620.10950046928772553No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4620.10950046928772553No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4590.10878942727936367No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4550.10784137126821454No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA4530.10736734326263996No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4500.10665630125427811No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4480.10618227324870352No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT4460.10570824524312897No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4450.10547123124034166No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA4430.1049972032347671No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4410.10452317522919254No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4400.10428616122640526No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4370.1035751192180434No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA4340.10286407720968155No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTC4340.10286407720968155No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCA4310.10215303520131969No Hit
CTTTACCACAGGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAA4300.10191602119853241No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTT4260.10096796518738328No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4260.10096796518738328No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTC4230.10025692317902142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)