FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628316
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT34780.5535431216139649No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG32090.5107302694822351No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23620.3759254897217324No Hit
GTCTATAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAC20170.3210168131959078No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC20040.31894779060218104No Hit
CTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT19310.30732943296048487No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT18480.2941195194774604No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA17380.27661240522284963No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA15630.2487601779996053No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT15040.2393699985357686No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA14480.23045728582433045No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT14400.22918404115126784No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT14040.22345444012248614No Hit
ATCCTAATCTCACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13890.22106710636049376No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13860.2205896396080953No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA13860.2205896396080953No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13740.21867977259850138No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG13720.21836146143023571No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG13550.21565581649997773No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG12150.19337403472138223No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG12140.1932148791372494No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11600.18462047759407685No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11530.1835063885051471No Hit
GGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA11450.1822331438320845No Hit
CACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG11400.18143736591142037No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG11390.18127821032728755No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT11340.18048243240662343No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA11330.18032327682249058No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA11290.1796866544859593No Hit
GGAACATATTGTGCTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTCA11230.17873172098116236No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10880.17316127553651348No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10680.16997816385385697No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC10570.1682274524283959No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA10380.16520349632987225No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA10280.16361194048854397No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC10200.16233869581548138No Hit
CGCATGCGCCGATGGCCTAATGCCAAGCCCCATCCAGTGGGGGTTGCGTC10190.16217954023134856No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA10150.16154291789481726No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT10090.1605879843900203No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT10090.1605879843900203No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10080.1604288288058875No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG10080.1604288288058875No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA10010.1593147397169577No Hit
CATGTATACAAGCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9900.15756402829149663No Hit
CTCTTACAGTTAATGAAAAAAGAAGATTAAAATTAATTATGCCTGCTAGG9780.15565416128190274No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT9570.1523118940151134No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC9350.14881047116419127No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9170.14594567064980044No Hit
ACACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG9160.1457865150656676No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA9020.14355833688780806No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG9000.1432400257195424No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA8980.14292171455127675No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8750.13926113611622176No Hit
CTCATAAGGTGGGAAACTTTACCGGGCTTTATTCTTCTACTGTACCTGTC8680.138147047027292No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC8650.13766958027489354No Hit
GTGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC8580.13655549118596375No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG8490.1351230909287683No Hit
CTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT8400.1336906906715729No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8310.13225829041437748No Hit
TTGACATACTTTCCAATCAATAGGTCTATTGACAGGCAGTTTCCGAAAAC8250.13130335690958053No Hit
TCCTAGTACAAAGACCTTCAACCTAATCTCCTCCCCCAACTCCTCCCAGT8220.13082589015718205No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA8210.13066673457304923No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8150.12971180106825228No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA8100.12891602314758815No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8040.1279610896427912No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTGAGATT8040.1279610896427912No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA8030.1278019340586584No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCGGTAAAGTTTCCCAC7870.1252554447125332No Hit
GTTTTATCCTAATCTCACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7830.12461882237600189No Hit
GGATAAGGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT7820.12445966679186905No Hit
ACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG7660.12191317744574386No Hit
GTACAAAGACCTTCAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA7620.12127655510921256No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7480.11904837693135302No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7440.11841175459482171No Hit
CAATAGGTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGC7230.1150694873280324No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7130.11347793148670414No Hit
GCCTTTATCCAAGGGCAAATATTTGGTGAGATTAGGATAAAACCTAGCAG7040.11204553122950872No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6990.11124975330884458No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG6970.11093144214057896No Hit
GATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACTT6950.1106131309723133No Hit
GTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCCTG6920.11013566421991482No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTCTTCTACTG6860.10918073071511786No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT6750.10743001928965679No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6740.10727086370552397No Hit
GCCATGGGAAGGATGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6730.10711170812139113No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTC6730.10711170812139113No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6710.10679339695312551No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6690.10647508578485985No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC6630.1055201522800629No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA6610.10520184111179724No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6590.1048835299435316No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTCCT6550.1042469076070003No Hit
GTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGCACAATA6520.10376944085460182No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA6470.10297366293393768No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTCAACCTAATCTCCTCC6440.10249619618153923No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC6430.1023370405974064No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTC6410.10201872942914075No Hit
GGTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGCACAAT6320.10058632917194534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)