FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628316
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG35390.5632516122460672No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT32740.5210753824508686No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23590.37544802296933394No Hit
GTCTATAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAC21220.3377281495298544No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT20640.32849712565015055No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19040.3030322321888986No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA17240.2743842270449901No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT15740.2505108894250664No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT15450.24589537748521445No Hit
CTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT15170.24143902112949536No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA14240.2266375518051426No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA14240.2266375518051426No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT13890.22106710636049376No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG13680.21772483909370446No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG13350.21247270481732122No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA13280.21135861572839143No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13140.2091304375505319No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG13090.20833465962986777No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT12730.20260505860108607No Hit
ATCCTAATCTCACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12640.20117265834389067No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC12300.1957613684833746No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12210.1943289682261792No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG12020.19130501212765552No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA11830.18828105602913184No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA11010.17523029813024021No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11000.17507114254610737No Hit
GGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA10980.17475283137784173No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA10900.17347958670477914No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA10550.16790914126013023No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10440.16615842983466916No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10400.16552180749813789No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG10370.16504434074573943No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG10300.16393025165680963No Hit
GGAACATATTGTGCTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTCA10160.16170207347895008No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA10050.159951362053489No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9960.1585189617962936No Hit
CTCTTACAGTTAATGAAAAAAGAAGATTAAAATTAATTATGCCTGCTAGG9680.15406260544057448No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC9470.15072033817378516No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9460.15056118258965234No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT9380.14928793791658973No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA9330.14849215999592563No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA9160.1457865150656676No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT9150.14562735948153477No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC9100.14483158156087064No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT8990.14308087013540957No Hit
GTTTTATCCTAATCTCACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8950.14244424779887827No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8830.14053438078928437No Hit
CACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG8420.13400900183983855No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA8410.13384984625570573No Hit
GTGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC8380.13337237950330727No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8320.1324174459985103No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA8280.13178082366197902No Hit
CATGTATACAAGCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8240.1311442013254477No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8220.13082589015718205No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8180.13018926782065074No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCGGTAAAGTTTCCCAC8160.12987095665238513No Hit
GGATAAGGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT8100.12891602314758815No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA8060.12827940081105685No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7990.12716531172212708No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7820.12445966679186905No Hit
GCCTTTATCCAAGGGCAAATATTTGGTGAGATTAGGATAAAACCTAGCAG7810.12430051120773623No Hit
ACACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG7710.12270895536640798No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7690.12239064419814233No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7640.12159486627747822No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA7600.12095824394094691No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTGAGATT7590.12079908835681409No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA7590.12079908835681409No Hit
CTCATAAGGTGGGAAACTTTACCGGGCTTTATTCTTCTACTGTACCTGTC7570.12048077718854844No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7530.11984415485201713No Hit
GTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAC7470.11888922134722019No Hit
CGCATGCGCCGATGGCCTAATGCCAAGCCCCATCCAGTGGGGGTTGCGTC7290.11602442083282934No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTCTTCTACTG7270.11570610966456368No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7230.1150694873280324No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7050.11220468681364154No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6980.11109059772471178No Hit
GTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGCACAATA6980.11109059772471178No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6910.109976508635782No Hit
CTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT6820.10854410837858658No Hit
CAATAGGTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGC6800.10822579721032093No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6730.10711170812139113No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA6690.10647508578485985No Hit
TCCTAGTACAAAGACCTTCAACCTAATCTCCTCCCCCAACTCCTCCCAGT6620.10536099669593006No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6570.10456521877526595No Hit
TTGACATACTTTCCAATCAATAGGTCTATTGACAGGCAGTTTCCGAAAAC6480.10313281851807052No Hit
GGTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGCACAAT6470.10297366293393768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)