FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388574
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGACAGTCCAA15890.4089311173676056No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13830.3559167623155435No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT13780.3546300061249595No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG13660.3515417912675578No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13400.3448506590765208No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTC11740.3021303535491309No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11480.295439221358094No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT11060.2846304693571881No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA10650.274079068594399No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9470.24371162249661582No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9330.24010870516298055No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9040.23264551925759316No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8960.2305867093526587No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8930.22981465563830827No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8820.2269837920190234No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8750.22518233335220578No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA8390.21591768878000073No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCATCATACGGCACTCAGGCA8170.21025596154143097No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8000.20588099049344524No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7970.20510893677909486No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7930.20407953182662764No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7810.20099131696922592No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7770.1999619120167587No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7600.19558694096877302No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7440.19146932115890408No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7170.1845208377297503No Hit
GTATAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG7060.18168997411046545No Hit
GTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTTCACCTCATCATACGG6950.17885911049118058No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6790.17474149068131167No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6600.16985181715709236No Hit
CTGTTATACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGCCTCCCC6490.16702095353780747No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6320.16264598248982176No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6260.16110187506112092No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6100.15698425525125204No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6040.15544014782255117No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6000.15441074287008394No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5950.15312398667949992No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5930.1526092842032663No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5860.15080782553644867No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5700.14669020572657976No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5640.14514609829787892No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5570.14334463963106128No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5550.14282993715482767No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5470.14077112724989319No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5430.13974172229742596No Hit
CTCTTGGACTGTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5420.13948437105930916No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5420.13948437105930916No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5380.13845496610684194No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5330.13716820991625792No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5300.13639615620190748No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5170.133050590106389No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5170.133050590106389No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5170.133050590106389No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5140.13227853639203857No Hit
CTTCTAAGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCT4990.12841826782028648No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT4980.1281609165821697No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4940.12713151162970246No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4930.12687416039158564No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4930.12687416039158564No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTTCACCTCATCA4910.12635945791535202No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4850.1248153504866512No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4830.12430064801041758No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4790.12327124305795034No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4790.12327124305795034No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCAAGACCTTTAACCT4790.12327124305795034No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4710.1212124331530159No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4580.11786686705749741No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4520.11632275962879658No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4500.11580805715256297No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4490.11555070591444615No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACAGGAACCTGCCC4470.11503600343821255No Hit
GTCCAAGAGTCCTCTTATGCAAGACAGTCCAAGAGTCCTCTTATGCAAGA4450.11452130096197893No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4440.11426394972386211No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA4350.11194778858081085No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4340.11169043734269406No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGACAGTCCAAGAGTCCTCTTATGC4310.11091838362834364No Hit
CCACAATAGTTGTCTGATTTTTAGGCCCATATTAACGTTGACATAGCTGA4300.11066103239022683No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC4220.10860222248529237No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4200.10808752000905876No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4200.10808752000905876No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4190.10783016877094197No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4190.10783016877094197No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4160.10705811505659153No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4150.10680076381847474No Hit
ACCTTGGGCAGGTTCCTGTGGGCGTTCACGGTGGTCTCCATGCGACGTGC4140.10654341258035792No Hit
CCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTTCACCT4090.10525665638977388No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4050.10422725143730666No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4020.10345519772295625No Hit
ATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTCACGGTGGTCTCCATGC4020.10345519772295625No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG3990.10268314400860583No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG3990.10268314400860583No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA3970.10216844153237221No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3960.1019110902942554No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC3920.10088168534178818No Hit
GCTTTATTCTTCTAAGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT3910.10062433410367139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)