FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388574
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGACAGTCCAA13950.35900497717294516No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG13820.3556594110774267No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12540.3227184525984755No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12110.31165234935945274No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12050.3101082419307519No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT12050.3101082419307519No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10790.2776819859280343No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10030.258123291831157No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9310.2395940026867469No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9150.23547638287687803No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTC9000.23161611430512594No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8420.21668974249435113No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8400.21617504001811752No Hit
CTGTTATACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGCCTCCCC8380.21566033754188393No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCATCATACGGCACTCAGGCA8340.2146309325894167No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC8200.2110280152557814No Hit
GTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTTCACCTCATCATACGG8160.20999861030331418No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7780.2002192632548755No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7650.19687369715935704No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7620.1961016434450066No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7610.19584429220688981No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7550.19430018477818894No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7520.19352813106383857No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7490.19275607734948813No Hit
GTATAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG7260.18683699887280159No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA7120.1832340815391663No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6830.1757708956337789No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6740.17345473449072762No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6490.16702095353780747No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6390.1644474411566394No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6080.15646955277501842No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6060.15595485029878478No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6010.15466809410820076No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5970.15363868915573353No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5690.14643285448846294No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5680.14617550325034614No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5620.1446313958216453No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5610.14437404458352848No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5570.14334463963106128No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5490.1412858297261268No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5450.14025642477365957No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5370.13819761486872514No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5320.1369108586781411No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5310.1366535074400243No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5150.1325358876301554No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5110.13150648267768816No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5080.13073442896333773No Hit
CTCTTGGACTGTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5030.1294476727727537No Hit
CTTCTAAGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCT4940.12713151162970246No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4850.1248153504866512No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4840.12455799924853438No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT4840.12455799924853438No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTTCACCTCATCA4830.12430064801041758No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCAAGACCTTTAACCT4790.12327124305795034No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4790.12327124305795034No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4730.12172713562924951No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4700.1209550819148991No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4680.12044037943866547No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4650.11966832572431506No Hit
CCACAATAGTTGTCTGATTTTTAGGCCCATATTAACGTTGACATAGCTGA4610.11863892077184784No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4610.11863892077184784No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4600.11838156953373102No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4550.11709481334314699No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4530.11658011086691338No Hit
GGCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC4510.11606540839067976No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4510.11606540839067976No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4500.11580805715256297No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4490.11555070591444615No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA4480.11529335467632935No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC4470.11503600343821255No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4430.11400659848574532No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4420.1137492472476285No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4410.1134918960095117No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4380.11271984229516128No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC4350.11194778858081085No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4320.11117573486646044No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4300.11066103239022683No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4290.11040368115211002No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACAGGAACCTGCCC4260.10963162743775962No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4230.10885957372340918No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4230.10885957372340918No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4220.10860222248529237No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4200.10808752000905876No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4120.10602871010412432No Hit
GTTATACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGCCTCCCCGT4110.1057713588660075No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4090.10525665638977388No Hit
CCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTTCACCT4040.10396990019918986No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC4000.10294049524672262No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA3950.1016537390561386No Hit
GCTTTATTCTTCTAAGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT3900.10036698286555457No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT3890.10010963162743775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)