FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379866
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12910.3398566863051708No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12520.3295899080201966No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10270.2703584948376533No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10000.26325072525574805No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA8910.23455639620287155No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8530.2245528686431531No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7870.20717832077627374No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7750.20401931207320476No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7680.20217655699641454No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7410.19506878741450934No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7040.18532851058004665No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6740.1774309888223742No Hit
CTCTTACAGTTAATGAGAAAAGAAGATTAAAATTAATTATGCCTGCTAGG6510.171376222141492No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6500.17111297141623624No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6490.1708497206909805No Hit
ACATAGAGGTGCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG6460.17005996851521327No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAGAAA6350.16716421053740002No Hit
CCTAATACAAAGATCATTAACCTAATCTCCTCCCCCGACTCCTCCCAGTC6100.16058294240600635No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCTTAT6090.16031969168075058No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5930.1561076800766586No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC5890.15505467717563562No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5730.15084266557154366No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5660.14899991049475342No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5570.14663065396745167No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5470.1439981467148942No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA5370.1413656394623367No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5330.1403126365613137No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5280.13899638293503497No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA5270.13873313220977923No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG5150.13557412350671028No Hit
GCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTG5130.13504762205619877No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5100.13425786988043154No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGCACCTCTATG5090.13399461915517577No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC5060.13320486697940853No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT5050.13294161625415277No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA4980.13109886117736252No Hit
GGATAATAAGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT4980.13109886117736252No Hit
CCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG4960.13057235972685105No Hit
TTCTATGTCAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCA4930.1297826075510838No Hit
GTTGTCAATATGCCCTGAGCCTGTGGGCTCCACCCCAAAAGGCCGCCGTG4920.12951935682582805No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4860.12793985247429357No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4820.12688684957327057No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4800.1263603481227591No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4800.1263603481227591No Hit
CTACTGTACCTGTCTTTAATTCTGAGTGGCAAACTCCCTCCTTTCCTCAC4790.12609709739750333No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4740.1247808437712246No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4720.12425434232071308No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4650.12241158724392286No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTTCTTTTCTCA4630.12188508579341135No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4610.12135858434289987No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4590.12083208289238836No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC4550.11977907999136538No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGT4510.11872607709034239No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4490.1181995756398309No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4450.11714657273880791No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4450.11714657273880791No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT4440.11688332201355216No Hit
CTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4390.1155670683872734No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4390.1155670683872734No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4360.11477731621150615No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4350.11451406548625041No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4310.11346106258522742No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC4180.1100388031569027No Hit
TTATCGGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA4140.10898580025587971No Hit
GGTTGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC4040.10635329300332222No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA4020.10582679155281072No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC3970.10451053792653198No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTTGGCCGGAACGG3930.103457535025509No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG3910.1029310335749975No Hit
CTCCCATAGGAGTCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3850.10135152922346302No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG3830.10082502777295152No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA3800.10003527559718428No Hit
CAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA3800.10003527559718428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)