FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379866
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13680.3601269921498634No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10830.28510053545197517No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10710.28194152674890616No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10140.2669362354093286No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9570.2519309440697509No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA8920.2348196469281273No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7280.1916465279861846No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7150.18822426855785987No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7040.18532851058004665No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6700.17637798592135123No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6340.16690095981214428No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6330.16663770908688852No Hit
CTCTTACAGTTAATGAGAAAAGAAGATTAAAATTAATTATGCCTGCTAGG5980.15742393370293734No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5950.1566341815271701No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5940.15637093080191436No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAGAAA5860.15426492499986838No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG5700.1500529133957764No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA5600.1474204061432189No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCTTAT5570.14663065396745167No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5550.14610415251694017No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5500.14478789889066146No Hit
ACATAGAGGTGCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5470.1439981467148942No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5380.14162889018759248No Hit
CCTAATACAAAGATCATTAACCTAATCTCCTCCCCCGACTCCTCCCAGTC5320.14004938583605797No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5270.13873313220977923No Hit
CCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG5250.13820663075926773No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5210.13715362785824475No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5130.13504762205619877No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5110.13452112060568727No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5090.13399461915517577No Hit
GGATAATAAGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT5070.1334681177046643No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5030.13241511480364127No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5010.1318886133531298No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC4930.1297826075510838No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4890.1287296046500608No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT4880.12846635392480507No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4870.1282031031995493No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGCACCTCTATG4810.12662359884801483No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4670.12293808869443434No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4550.11977907999136538No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC4540.11951582926610962No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA4480.11793632491457515No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTTCTTTTCTCA4470.1176730741893194No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG4470.1176730741893194No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCAACCACG4450.11714657273880791No Hit
CTACTGTACCTGTCTTTAATTCTGAGTGGCAAACTCCCTCCTTTCCTCAC4440.11688332201355216No Hit
GTTGTCAATATGCCCTGAGCCTGTGGGCTCCACCCCAAAAGGCCGCCGTG4420.11635682056304066No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4410.11609356983778492No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4410.11609356983778492No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA4380.11530381766201765No Hit
GGTTGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC4350.11451406548625041No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4330.11398756403573891No Hit
CTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4310.11346106258522742No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC4270.11240805968420443No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4220.1110918060579257No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC4220.1110918060579257No Hit
TTCTATGTCAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCA4210.11082855533266994No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4100.10793279735485672No Hit
CTCCCATAGGAGTCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4090.10766954662960097No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4060.1068797944538337No Hit
TTCCCCCACTGTTTGGTTTTCAGTTATGTTGATGATGTGGTATTGGGGGC4030.10609004227806647No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4010.10556354082755498No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4000.10530029010229923No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT3970.10451053792653198No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT3970.10451053792653198No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT3940.10372078575076474No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGT3900.10266778284974175No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA3890.102404532124486No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA3860.10161477994871876No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC3820.10056177704769577No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT3820.10056177704769577No Hit
GCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTG3810.10029852632244002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)